High-density lipoprotein associated with secondary vitellogenesis in the hemolymph of the crayfish Cherax quadricarinatus

Galit Yehezkel, Reuben Chayoth, Uri Abdu, Isam Khalaila, Amir Sagi

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The high-density lipoproteins LPI and LPII were isolated from the hemolymph of the crayfish Cherax quadricarinatus by gradient ultracentrifugation and high-performance liquid chromatography (HPLC). Both lipoproteins contained a carotenoid moiety. LPI is comprised of a single polypeptide with an approximate molecular mass of 96 kDa. LPII was composed of two similar native components, LPIIa and LPIIb, both having polypeptides of 80 and 177 kDa. Both under natural conditions and after endocrine manipulations, LPI was present in males and in females, regardless of the female reproductive stage. LPII was present only in secondary-vitellogenic females, but not during the winter reproductive arrest period. LPII was also absent from young females that had received androgenic gland implants. LPII also appeared in the hemolymph of intersex individuals from which the androgenic gland had been removed. It is therefore suggested that LPII serves as a marker indicating the onset of secondary vitellogenesis in C. quadricarinatus females. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)411-421
Number of pages11
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume127
Issue number3
DOIs
StatePublished - 1 Jan 2000

Keywords

  • Androgenic gland
  • Cherax quadricarinatus
  • Crustacea
  • Decapoda
  • HDL
  • Hemolymphatic lipoproteins
  • Reproduction
  • Vitellogenesis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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