High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents

Boris Polyak; Ilia Fishbein; Michael Chorny; Ivan Alferiev; Darryl Williams; Ben Yellen; Gary Friedman; Robert J. Levy

doi:10.1073/pnas.0708338105

High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents

Boris Polyak, Ilia Fishbein, Michael Chorny, Ivan Alferiev, Darryl Williams, Ben Yellen, Gary Friedman, Robert J. Levy

Research output: Contribution to journal › Article › peer-review

246 Scopus citations

Abstract

A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

Original language	English
Pages (from-to)	698-703
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	2
DOIs	https://doi.org/10.1073/pnas.0708338105
State	Published - 15 Jan 2008
Externally published	Yes

Keywords

Cell therapy
Gene therapy
Local delivery
Nanotechnology

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.0708338105

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title = "High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents",

abstract = "A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.",

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author = "Boris Polyak and Ilia Fishbein and Michael Chorny and Ivan Alferiev and Darryl Williams and Ben Yellen and Gary Friedman and Levy, {Robert J.}",

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AU - Polyak, Boris

AU - Fishbein, Ilia

AU - Chorny, Michael

AU - Alferiev, Ivan

AU - Williams, Darryl

AU - Yellen, Ben

AU - Friedman, Gary

AU - Levy, Robert J.

PY - 2008/1/15

Y1 - 2008/1/15

N2 - A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

AB - A cell delivery strategy was investigated that was hypothesized to enable magnetic targeting of endothelial cells to the steel surfaces of intraarterial stents because of the following mechanisms: (i) preloading cells with biodegradable polymeric superparamagnetic nanoparticles (MNPs), thereby rendering the cells magnetically responsive; and (ii) the induction of both magnetic field gradients around the wires of a steel stent and magnetic moments within MNPs because of a uniform external magnetic field, thereby targeting MNP-laden cells to the stent wires. In vitro studies demonstrated that MNP-loaded bovine aortic endothelial cells (BAECs) could be magnetically targeted to steel stent wires. In vivo MNP-loaded BAECs transduced with adenoviruses expressing luciferase (Luc) were targeted to stents deployed in rat carotid arteries in the presence of a uniform magnetic field with significantly greater Luc expression, detected by in vivo optical imaging, than nonmagnetic controls.

KW - Cell therapy

KW - Gene therapy

KW - Local delivery

KW - Nanotechnology

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High field gradient targeting of magnetic nanoparticle-loaded endothelial cells to the surfaces of steel stents

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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