High-sensitivity and high-specificity biomechanical imaging by stimulated Brillouin scattering microscopy

Itay Remer, Roni Shaashoua, Netta Shemesh, Anat Ben-Zvi, Alberto Bilenca

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Label-free, non-contact imaging with mechanical contrast and optical sectioning is a substantial challenge in microscopy. Spontaneous Brillouin scattering microscopy meets this challenge, but encounters a trade-off between acquisition speed and the specificity for biomechanical constituents with overlapping Brillouin bands. Stimulated Brillouin scattering microscopy overcomes this trade-off and enables the cross-sectional imaging of live Caenorhabditis elegans at the organ and subcellular levels, with both elasticity and viscosity contrasts at high specificity and with practical recording times.

Original languageEnglish
Pages (from-to)913-916
Number of pages4
JournalNature Methods
Volume17
Issue number9
DOIs
StatePublished - 1 Sep 2020

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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