High throughput screen for the improvement of inducible promoters for tumor microenvironment cues

Omri Sharabi, Yariv Greenshpan, Noa Ofir, Aner Ottolenghi, Tamar Levi, Leonid Olender, Zachor Adler-Agmon, Angel Porgador, Roi Gazit

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cancer immunotherapies are highly potent and are gaining wide clinical usage. However, severe side effects require focusing effector immune cell activities on the tumor microenvironment (TME). We recently developed a chimeric antigen receptor tumor-induced vector (CARTIV), a synthetic promoter activated by TME factors. To improve CARTIV functions including background, activation levels, and synergism, we screened a library of promoters with variations in key positions. Here, we present a screening method involving turning ON/OFF stimulating TNFα and IFNγ cytokines, followed by sequential cell sorting. Sequencing of enriched promoters identified seventeen candidates, which were cloned and whose activities were then validated, leading to the identification of two CARTIVs with lower background and higher induction. We further combined a third hypoxia element with the two-factor CARTIV, demonstrating additional modular improvement. Our study presents a method of fine-tuning synthetic promoters for desired immunotherapy needs.

Original languageEnglish
Article number7169
Number of pages12
JournalScientific Reports
Volume12
DOIs
StatePublished - 3 May 2022

Keywords

  • Cues
  • Gene Library
  • Humans
  • Immunotherapy
  • Neoplasms
  • Promoter Regions, Genetic
  • Tumor Microenvironment/genetics

ASJC Scopus subject areas

  • General

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