High-throughput screens and selections of enzyme-encoding genes

Amir Aharoni, Andrew D. Griffiths, Dan S. Tawfik

Research output: Contribution to journalReview articlepeer-review

153 Scopus citations

Abstract

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume9
Issue number2
DOIs
StatePublished - 1 Jan 2005
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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