High-throughput screens and selections of enzyme-encoding genes

Amir Aharoni; Andrew D. Griffiths; Dan S. Tawfik

doi:10.1016/j.cbpa.2005.02.002

High-throughput screens and selections of enzyme-encoding genes

Amir Aharoni, Andrew D. Griffiths, Dan S. Tawfik

Research output: Contribution to journal › Review article › peer-review

156 Scopus citations

Abstract

The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>10⁶ gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

Original language	English
Pages (from-to)	210-216
Number of pages	7
Journal	Current Opinion in Chemical Biology
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.cbpa.2005.02.002
State	Published - 1 Jan 2005
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

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10.1016/j.cbpa.2005.02.002

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title = "High-throughput screens and selections of enzyme-encoding genes",

abstract = "The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.",

author = "Amir Aharoni and Griffiths, {Andrew D.} and Tawfik, {Dan S.}",

note = "Funding Information: We acknowledge financial support to DST by the Israel Science Foundation through its Bikura program, and to both ADG and DST by the European Union through the ENDIRPRO training network. DST is the incumbent of the Elaine Blond Career Development Chair.",

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AU - Griffiths, Andrew D.

AU - Tawfik, Dan S.

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N2 - The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

AB - The availability of vast gene repertoires from both natural sources (genomic and cDNA libraries) and artificial sources (gene libraries) demands the development and application of novel technologies that enable the screening or selection of large libraries for a variety of enzymatic activities. We describe recent developments in the selection of enzyme-coding genes for directed evolution and functional genomics. We focus on HTS approaches that enable selection from large libraries (>106 gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular.

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JO - Current Opinion in Chemical Biology

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High-throughput screens and selections of enzyme-encoding genes

Abstract

ASJC Scopus subject areas

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