Hippuric acid synthesizing system during development. Kinetic studies and inhibition with salicylic acid

Rafael Gorodischer; Joseph Krasner; Sumner J. Yaffe

doi:10.1016/0006-2952(71)90472-2

Hippuric acid synthesizing system during development. Kinetic studies and inhibition with salicylic acid

Rafael Gorodischer, Joseph Krasner, Sumner J. Yaffe

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

p-Aminohippurate synthesis was investigated in mouse liver suspensions. It was found to be an age-dependent reaction with minimal activity in the neonatal period and adult values attained at 1 month of age. Sodium salicylate strongly inhibited the reaction; this inhibition was not suppressed by K or Mg ions. Kinetic studies indicated that the salicylate inhibition of p-aminohippurate synthesis is competitive in nature. Developmental kinetic experiments disclosed slightly different K_m values in young versus adult animals; however, K_i values at 7 and 68 days differed markedly.

Original language	English
Pages (from-to)	67-72
Number of pages	6
Journal	Biochemical Pharmacology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/0006-2952(71)90472-2
State	Published - 1 Jan 1971
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Pharmacology

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10.1016/0006-2952(71)90472-2

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title = "Hippuric acid synthesizing system during development. Kinetic studies and inhibition with salicylic acid",

abstract = "p-Aminohippurate synthesis was investigated in mouse liver suspensions. It was found to be an age-dependent reaction with minimal activity in the neonatal period and adult values attained at 1 month of age. Sodium salicylate strongly inhibited the reaction; this inhibition was not suppressed by K or Mg ions. Kinetic studies indicated that the salicylate inhibition of p-aminohippurate synthesis is competitive in nature. Developmental kinetic experiments disclosed slightly different Km values in young versus adult animals; however, Ki values at 7 and 68 days differed markedly.",

author = "Rafael Gorodischer and Joseph Krasner and Yaffe, {Sumner J.}",

note = "Funding Information: l This research was supported in part by Public Health Service Grants FR-05493, HD-04287, RR-77 and a grant from the United Health Foundation of Western New York. 67",

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T1 - Hippuric acid synthesizing system during development. Kinetic studies and inhibition with salicylic acid

AU - Gorodischer, Rafael

AU - Krasner, Joseph

AU - Yaffe, Sumner J.

N1 - Funding Information: l This research was supported in part by Public Health Service Grants FR-05493, HD-04287, RR-77 and a grant from the United Health Foundation of Western New York. 67

PY - 1971/1/1

Y1 - 1971/1/1

N2 - p-Aminohippurate synthesis was investigated in mouse liver suspensions. It was found to be an age-dependent reaction with minimal activity in the neonatal period and adult values attained at 1 month of age. Sodium salicylate strongly inhibited the reaction; this inhibition was not suppressed by K or Mg ions. Kinetic studies indicated that the salicylate inhibition of p-aminohippurate synthesis is competitive in nature. Developmental kinetic experiments disclosed slightly different Km values in young versus adult animals; however, Ki values at 7 and 68 days differed markedly.

AB - p-Aminohippurate synthesis was investigated in mouse liver suspensions. It was found to be an age-dependent reaction with minimal activity in the neonatal period and adult values attained at 1 month of age. Sodium salicylate strongly inhibited the reaction; this inhibition was not suppressed by K or Mg ions. Kinetic studies indicated that the salicylate inhibition of p-aminohippurate synthesis is competitive in nature. Developmental kinetic experiments disclosed slightly different Km values in young versus adult animals; however, Ki values at 7 and 68 days differed markedly.

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DO - 10.1016/0006-2952(71)90472-2

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SN - 0006-2952

VL - 20

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JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 1

ER -

Hippuric acid synthesizing system during development. Kinetic studies and inhibition with salicylic acid

Abstract

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