Histochemical and Histofluorescence Tracing of Chelatable Zinc in the Developing Mouse

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Zinc is an essential element in mammalian development. However, little is known about concentrations of zinc in specific regions/organs in the embryo. We have employed selenite autometallography (AMG) and TSQ histofluoroscence to detect histochemically reactive (chelatable) zinc in whole midsagittal embryos and sections from neonatal mice. Chelatable zinc exhibited a broad distribution, being particularly localized to rapidly proliferating tissues, such as skin and gastrointestinal epithelium. Zinc was also observed in various types of tissues such as bone and liver. In the perinatal central nervous system, zinc was present almost exclusively in choroid plexus. The two methods used demonstrated generally similar distributions with some exceptions, e.g., in liver and blood. The ubiquity of zinc in the embryo, particularly in rapidly proliferating tissues, suggests a widespread role in fetal physiology.

Original languageEnglish
Pages (from-to)529-539
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Issue number4
StatePublished - 1 Jan 2004


  • Embryo
  • Gestation
  • Localization
  • Metal
  • Tissue

ASJC Scopus subject areas

  • Anatomy
  • Histology


Dive into the research topics of 'Histochemical and Histofluorescence Tracing of Chelatable Zinc in the Developing Mouse'. Together they form a unique fingerprint.

Cite this