Histologic validation of auto-contoured dominant intraprostatic lesions on [18F] DCFPyL PSMA-PET imaging

Ryan Alfano, Glenn S. Bauman, Wei Liu, Jonathan D. Thiessen, Irina Rachinsky, William Pavlosky, John Butler, Mena Gaed, Madeleine Moussa, Jose A. Gomez, Joseph L. Chin, Stephen Pautler, Aaron D. Ward

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: PSMA-PET1 has shown good concordance with histology, but there is a need to investigate the ability of PSMA-PET to delineate DIL2 boundaries for guided biopsy and focal therapy planning. Objective: To determine threshold and margin combinations that satisfy the following criteria: ≥95% sensitivity with max specificity and ≥95% specificity with max sensitivity. Design, setting and participants: We registered pathologist-annotated whole-mount mid-gland prostatectomy histology sections cut in 4.4 mm intervals from 12 patients to pre-surgical PSMA-PET/MRI by mapping histology to ex-vivo imaging to in-vivo imaging. We generated PET-derived tumor volumes using boundaries defined by thresholded PET volumes from 1–100% of SUV3max in 1% intervals. At each interval, we applied margins of 0–30 voxels in one voxel increments, giving 3000 volumes/patient. Outcome measurements: Mean and standard deviation of sensitivity and specificity for cancer detection within the 2D oblique histologic planes that intersected with the 3D PET volume for each patient. Results and limitations: A threshold of 67% SUV max with an 8.4 mm margin achieved a (mean ± std.) sensitivity of 95.0 ± 7.8% and specificity of 76.4 ± 14.7%. A threshold of 81% SUV max with a 5.1 mm margin achieved sensitivity of 65.1 ± 28.4% and specificity of 95.1 ± 5.2%. Conclusions: Preliminary evidence of thresholding and margin expansion of PSMA-PET images targeted at DILs validated with histopathology demonstrated excellent mean sensitivity and specificity in the setting of focal therapy/boosting and guided biopsy. These parameters can be used in a larger validation study supporting clinical translation.

Original languageEnglish
Pages (from-to)34-41
Number of pages8
JournalRadiotherapy and Oncology
StatePublished - 1 Nov 2020
Externally publishedYes


  • Dominant intraprostatic lesion
  • Focal boosting
  • Guided biopsy
  • Magnetic resonance imaging
  • Positron-emission tomography
  • Prostate cancer

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging


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