TY - JOUR
T1 - Hollow fiber enzymic reactors for a two substrate process
T2 - Analytical modeling and numerical simulations
AU - Salzman, Galia
AU - Tadmor, Rafael
AU - Guzy, Serge
AU - Sideman, Samuel
AU - Lotan, Noah
N1 - Funding Information:
This research was supported in part by grants from the Leonard and Diane Sherman Research Fund, Archie Micay Biomedical Research Fund, and from the Otto Mehyerhof Biotechnology Laboratory established at the Technion by the Minerva Foundation. All are gratefully acknowledged. One of the authors (GS) also acknowledges the support provided by the Miriam and Aaron Gutwirth Memorial Fellowship, the Julius and Dorothea Harband Fellowship, the Samuel Stassler Memorial Fellowship and the Mailman Foundation Fellowship.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - The use of immobilized enzyme reactors in biotechnology and biomedicine is rapidly expanding. This study concentrates on hollow-fiber (HF) enzymic reactors for continuous, single-pass operation. The enzyme, in a soluble form, is physically confined within the shell section of the reactor and the substrate solution flows through the lumen section of it. We consider here a two-substrate reaction proceeding via the Ping-Pong mechanism, with substrates and reaction products diffusing through the fiber wall. The developed analytical model enables to calculate the expected conversion as a function of the volumetric flow rate, kinetic constants, diffusion coefficients, geometric dimensions of the reactor, the flow regimen in the apparatus and substrates concentrations. The model equations are solved by a numerical procedure and the system performance is simulated. Depending on the operation conditions employed, the reactor is controlled by kinetic processes, diffusion processes, or both.
AB - The use of immobilized enzyme reactors in biotechnology and biomedicine is rapidly expanding. This study concentrates on hollow-fiber (HF) enzymic reactors for continuous, single-pass operation. The enzyme, in a soluble form, is physically confined within the shell section of the reactor and the substrate solution flows through the lumen section of it. We consider here a two-substrate reaction proceeding via the Ping-Pong mechanism, with substrates and reaction products diffusing through the fiber wall. The developed analytical model enables to calculate the expected conversion as a function of the volumetric flow rate, kinetic constants, diffusion coefficients, geometric dimensions of the reactor, the flow regimen in the apparatus and substrates concentrations. The model equations are solved by a numerical procedure and the system performance is simulated. Depending on the operation conditions employed, the reactor is controlled by kinetic processes, diffusion processes, or both.
KW - Analytical modeling
KW - Hollow fiber enzymic reactor
KW - Numerical simulations
UR - http://www.scopus.com/inward/record.url?scp=0033380783&partnerID=8YFLogxK
U2 - 10.1016/S0255-2701(99)00020-3
DO - 10.1016/S0255-2701(99)00020-3
M3 - Article
AN - SCOPUS:0033380783
SN - 0255-2701
VL - 38
SP - 289
EP - 299
JO - Chemical Engineering and Processing: Process Intensification
JF - Chemical Engineering and Processing: Process Intensification
IS - 4-6
ER -