Hopping around an entropic barrier created by force

Ronen Berkovich, Sergi Garcia-Manyes, Joseph Klafter, Michael Urbakh, Julio M. Fernández

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


We use Langevin dynamics to investigate the role played by the recently discovered force-induced entropic energy barrier on the two-state hopping phenomena that has been observed in single RNA, DNA and protein molecules placed under a stretching force. Simple considerations about the free energy of a molecule readily show that the application of force introduces an entropic barrier separating the collapsed state of the molecule, from a force-driven extended conformation. A notable characteristic of the force induced barrier is its long distances to transition state, up to tens of nanometers, which renders the kinetics of crossing this barrier highly sensitive to an applied force. Langevin dynamics across such force induced barriers readily demonstrates the hopping behavior observed for a variety of single molecules placed under force. Such hopping is frequently interpreted as a manifestation of two-state folding/unfolding reactions observed in bulk experiments. However, given that such barriers do not exist at zero force these reactions do not take place at all in bulk.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - 3 Dec 2010
Externally publishedYes


  • Force spectroscopy
  • Hopping
  • Langevin dynamics
  • Protein folding
  • Proteins
  • Single molecule

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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