TY - JOUR
T1 - Human aqueous humor phosphatase activity in cataract and glaucoma
AU - Latarya, Galina
AU - Mansour, Ahmad
AU - Epstein, Irena
AU - Cotlear, Daniel
AU - Pikkel, Joseph
AU - Levartovsky, Shmuel
AU - Yulish, Michael
AU - Beit-Yannai, Elie
PY - 2012/3/1
Y1 - 2012/3/1
N2 - PURPOSE. To investigate the presence and activity of protein phosphatase-2A (PPase2A), protein phosphatase-2C (PPase2C), and protein tyrosine phosphatases (PTPs) in the human aqueous humor (AH) of patients with primary open-angle glaucoma (POAG) and cataract and to study the correlation between these phosphatases and the redox state of the AH. METHODS. Eighty-six cataract patients and 29 POAG patients who were scheduled for cataract surgery with or without glaucoma surgery were enrolled in the study. PPase2A, PPase2C, and PTPs levels in AH were measured by enzymelinked immunosorbent assays, Western blot analyses, and spectral methods. The redox state was measured by spectral and fluorescent methods. RESULTS. Phosphatase activity-positive results were significantly higher in AH samples from the POAG group (PP2A χ 2(1) = 11.754, P < 0.01; PP2C χ 2(1) = 8.754, P < 0.01; PTP χ 2(1) = 11.073, P < 0.01). Western blot analysis revealed higher PP2C levels in the AH of glaucoma patients compared with PP2C levels in the AH of cataract patients (P = 0.012). Both oxidized/reduced glutathione ratios and superoxide dismutase levels in the AH were significantly higher in the glaucoma group than in the cataract group. Finally significant correlations were found between PP2A and PP2C, PP2A and PTP, and total antioxidant activity and PTP levels. CONCLUSIONS. There is a statistically significant difference between phosphatase levels in the AH of POAG patients and cataract patients. The phosphatase content of the AH represents tissue pathology, but their presence in the AH may be attributed to cell debris or to active signaling to other molecular events.
AB - PURPOSE. To investigate the presence and activity of protein phosphatase-2A (PPase2A), protein phosphatase-2C (PPase2C), and protein tyrosine phosphatases (PTPs) in the human aqueous humor (AH) of patients with primary open-angle glaucoma (POAG) and cataract and to study the correlation between these phosphatases and the redox state of the AH. METHODS. Eighty-six cataract patients and 29 POAG patients who were scheduled for cataract surgery with or without glaucoma surgery were enrolled in the study. PPase2A, PPase2C, and PTPs levels in AH were measured by enzymelinked immunosorbent assays, Western blot analyses, and spectral methods. The redox state was measured by spectral and fluorescent methods. RESULTS. Phosphatase activity-positive results were significantly higher in AH samples from the POAG group (PP2A χ 2(1) = 11.754, P < 0.01; PP2C χ 2(1) = 8.754, P < 0.01; PTP χ 2(1) = 11.073, P < 0.01). Western blot analysis revealed higher PP2C levels in the AH of glaucoma patients compared with PP2C levels in the AH of cataract patients (P = 0.012). Both oxidized/reduced glutathione ratios and superoxide dismutase levels in the AH were significantly higher in the glaucoma group than in the cataract group. Finally significant correlations were found between PP2A and PP2C, PP2A and PTP, and total antioxidant activity and PTP levels. CONCLUSIONS. There is a statistically significant difference between phosphatase levels in the AH of POAG patients and cataract patients. The phosphatase content of the AH represents tissue pathology, but their presence in the AH may be attributed to cell debris or to active signaling to other molecular events.
UR - http://www.scopus.com/inward/record.url?scp=84860652904&partnerID=8YFLogxK
U2 - 10.1167/iovs.11-9120
DO - 10.1167/iovs.11-9120
M3 - Article
C2 - 22323459
AN - SCOPUS:84860652904
SN - 0146-0404
VL - 53
SP - 1679
EP - 1684
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
IS - 3
ER -