Human placental Hofbauer cells express sprouty proteins: A possible modulating mechanism of villous branching

E. Y. Anteby; S. Natanson-Yaron; C. Greenfield; D. Goldman-Wohl; R. Haimov-Kochman; H. Holzer; S. Yagel

doi:10.1016/j.placenta.2004.08.008

Human placental Hofbauer cells express sprouty proteins: A possible modulating mechanism of villous branching

E. Y. Anteby, S. Natanson-Yaron, C. Greenfield, D. Goldman-Wohl, R. Haimov-Kochman, H. Holzer, S. Yagel

Research output: Contribution to journal › Article › peer-review

73 Scopus citations

Abstract

The development of the chorionic villous tree into a complex and organized ramified tubular network can be termed branching morphogenesis. Studying the molecular mechanisms involved in this process may contribute to the understanding of pregnancy complications such as preeclampsia. Sprouty (Spry) proteins are important regulators of branching morphogenesis and growth factor signaling. We analyzed the expression of Spry genes in human placenta. RT-PCR and immunohistochemistry were employed to detect placental Spry expression. Quantitative RT-PCR was used to assess the effect of FGF and reduced oxygen fraction on Spry gene expression. Spry 1, 2 and 3 expression was observed in placental tissue from all three trimesters. Our results reveal for the first time that Spry proteins are localized in the stroma of the chorionic villi, adjacent to cytotrophoblasts in areas of villous sprouting. Immunofluorescent double staining with anti-Spry and anti-CD68 confirmed that placental macrophages (Hofbauer cells) express Spry. Reduced oxygen fraction, FGF-4 and FGF-10 stimulated Spry-2 expression. Hofbauer cells also expressed c-Cbl, a protein that interacts with Spry. Placental expression of Spry and c-Cbl implies an important role for Hofbauer cells in placental development, possibly through a mesenchymal-epithelial interaction with trophoblasts. Regulation of Spry-2 expression by FGF-4 and FGF-10 suggests an orchestrated regulatory system that modulates villous branching.

Original language	English
Pages (from-to)	476-483
Number of pages	8
Journal	Placenta
Volume	26
Issue number	6
DOIs	https://doi.org/10.1016/j.placenta.2004.08.008
State	Published - 1 Jul 2005
Externally published	Yes

Keywords

Fibroblast growth factor
Hofbauer cells
Placenta
Sprouty
Trophoblast

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology
Developmental Biology

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10.1016/j.placenta.2004.08.008

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@article{37263c63e7c743c68870b0d84d98cb9d,

title = "Human placental Hofbauer cells express sprouty proteins: A possible modulating mechanism of villous branching",

abstract = "The development of the chorionic villous tree into a complex and organized ramified tubular network can be termed branching morphogenesis. Studying the molecular mechanisms involved in this process may contribute to the understanding of pregnancy complications such as preeclampsia. Sprouty (Spry) proteins are important regulators of branching morphogenesis and growth factor signaling. We analyzed the expression of Spry genes in human placenta. RT-PCR and immunohistochemistry were employed to detect placental Spry expression. Quantitative RT-PCR was used to assess the effect of FGF and reduced oxygen fraction on Spry gene expression. Spry 1, 2 and 3 expression was observed in placental tissue from all three trimesters. Our results reveal for the first time that Spry proteins are localized in the stroma of the chorionic villi, adjacent to cytotrophoblasts in areas of villous sprouting. Immunofluorescent double staining with anti-Spry and anti-CD68 confirmed that placental macrophages (Hofbauer cells) express Spry. Reduced oxygen fraction, FGF-4 and FGF-10 stimulated Spry-2 expression. Hofbauer cells also expressed c-Cbl, a protein that interacts with Spry. Placental expression of Spry and c-Cbl implies an important role for Hofbauer cells in placental development, possibly through a mesenchymal-epithelial interaction with trophoblasts. Regulation of Spry-2 expression by FGF-4 and FGF-10 suggests an orchestrated regulatory system that modulates villous branching.",

keywords = "Fibroblast growth factor, Hofbauer cells, Placenta, Sprouty, Trophoblast",

author = "Anteby, {E. Y.} and S. Natanson-Yaron and C. Greenfield and D. Goldman-Wohl and R. Haimov-Kochman and H. Holzer and S. Yagel",

note = "Funding Information: This work was supported in part by Israel's Ministry of Health, Chief Scientist Grant and by the Joint Hebrew University-Hadassah Grant.",

year = "2005",

month = jul,

day = "1",

doi = "10.1016/j.placenta.2004.08.008",

language = "English",

volume = "26",

pages = "476--483",

journal = "Placenta",

issn = "0143-4004",

publisher = "W.B. Saunders Ltd",

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TY - JOUR

T1 - Human placental Hofbauer cells express sprouty proteins

T2 - A possible modulating mechanism of villous branching

AU - Anteby, E. Y.

AU - Natanson-Yaron, S.

AU - Greenfield, C.

AU - Goldman-Wohl, D.

AU - Haimov-Kochman, R.

AU - Holzer, H.

AU - Yagel, S.

N1 - Funding Information: This work was supported in part by Israel's Ministry of Health, Chief Scientist Grant and by the Joint Hebrew University-Hadassah Grant.

PY - 2005/7/1

Y1 - 2005/7/1

N2 - The development of the chorionic villous tree into a complex and organized ramified tubular network can be termed branching morphogenesis. Studying the molecular mechanisms involved in this process may contribute to the understanding of pregnancy complications such as preeclampsia. Sprouty (Spry) proteins are important regulators of branching morphogenesis and growth factor signaling. We analyzed the expression of Spry genes in human placenta. RT-PCR and immunohistochemistry were employed to detect placental Spry expression. Quantitative RT-PCR was used to assess the effect of FGF and reduced oxygen fraction on Spry gene expression. Spry 1, 2 and 3 expression was observed in placental tissue from all three trimesters. Our results reveal for the first time that Spry proteins are localized in the stroma of the chorionic villi, adjacent to cytotrophoblasts in areas of villous sprouting. Immunofluorescent double staining with anti-Spry and anti-CD68 confirmed that placental macrophages (Hofbauer cells) express Spry. Reduced oxygen fraction, FGF-4 and FGF-10 stimulated Spry-2 expression. Hofbauer cells also expressed c-Cbl, a protein that interacts with Spry. Placental expression of Spry and c-Cbl implies an important role for Hofbauer cells in placental development, possibly through a mesenchymal-epithelial interaction with trophoblasts. Regulation of Spry-2 expression by FGF-4 and FGF-10 suggests an orchestrated regulatory system that modulates villous branching.

AB - The development of the chorionic villous tree into a complex and organized ramified tubular network can be termed branching morphogenesis. Studying the molecular mechanisms involved in this process may contribute to the understanding of pregnancy complications such as preeclampsia. Sprouty (Spry) proteins are important regulators of branching morphogenesis and growth factor signaling. We analyzed the expression of Spry genes in human placenta. RT-PCR and immunohistochemistry were employed to detect placental Spry expression. Quantitative RT-PCR was used to assess the effect of FGF and reduced oxygen fraction on Spry gene expression. Spry 1, 2 and 3 expression was observed in placental tissue from all three trimesters. Our results reveal for the first time that Spry proteins are localized in the stroma of the chorionic villi, adjacent to cytotrophoblasts in areas of villous sprouting. Immunofluorescent double staining with anti-Spry and anti-CD68 confirmed that placental macrophages (Hofbauer cells) express Spry. Reduced oxygen fraction, FGF-4 and FGF-10 stimulated Spry-2 expression. Hofbauer cells also expressed c-Cbl, a protein that interacts with Spry. Placental expression of Spry and c-Cbl implies an important role for Hofbauer cells in placental development, possibly through a mesenchymal-epithelial interaction with trophoblasts. Regulation of Spry-2 expression by FGF-4 and FGF-10 suggests an orchestrated regulatory system that modulates villous branching.

KW - Fibroblast growth factor

KW - Hofbauer cells

KW - Placenta

KW - Sprouty

KW - Trophoblast

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DO - 10.1016/j.placenta.2004.08.008

M3 - Article

AN - SCOPUS:20444414134

SN - 0143-4004

VL - 26

SP - 476

EP - 483

JO - Placenta

JF - Placenta

IS - 6

ER -

Human placental Hofbauer cells express sprouty proteins: A possible modulating mechanism of villous branching

Abstract

Keywords

ASJC Scopus subject areas

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