Hydrogels composed of hyaluronic acid and dendritic ELPs: Hierarchical structure and physical properties

Yulia Shmidov, Mingjun Zhou, Gal Yosefi, Ronit Bitton, John B. Matson

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Hydrogels that mimic the native extracellular matrix were prepared from hyaluronic acid (HA) and amine-terminated dendritic elastin-like peptides (denELPs) of generations 1, 2, and 3 (G1, 2, and 3) as crosslinking units. The physical properties of the hydrogels were investigated by rheology, scanning electron microscopy, swelling tests, small-angle X-ray scattering (SAXS), and model drug loading and release assays. Hydrogel properties depended on the generation number of the denELP, which contained structural segments based on the repeating GLPGL pentamer. Hydrogels with higher generation denELPs (G2 and 3) showed similar properties, but those prepared from G1 denELPs were rheologically weaker, had a larger mesh size, absorbed less model drug, and released the drug more quickly. Interestingly, most of the HA-denELP hydrogels studied here remained transparent upon gelation, but after lyophilization and addition of water retained opaque, "solid-like" regions for up to 4 d during rehydration. This rehydration process was carefully evaluated through time-course SAXS studies, and the phenomenon was attributed to the formation of pre-coacervates in the gel-forming step, which slowly swelled in water during rehydration. These findings provide important insights into the behavior of ELP-based hydrogels, in which physical crosslinking of the ELP domains can be controlled to tune mechanical properties, highlighting the potential of HA-denELP hydrogels as biomaterials.

Original languageEnglish
Pages (from-to)917-925
Number of pages9
JournalSoft Matter
Issue number5
StatePublished - 1 Jan 2019

ASJC Scopus subject areas

  • General Chemistry
  • Condensed Matter Physics


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