Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells

P. V. Avdonin; A. A. Tsitrina; G. Y. Mironova; P. P. Avdonin; I. L. Zharkikh; A. D. Nadeev; N. V. Goncharov

doi:10.1134/S106235901705003X

Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells

P. V. Avdonin, A. A. Tsitrina, G. Y. Mironova, P. P. Avdonin, I. L. Zharkikh, A. D. Nadeev, N. V. Goncharov

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H₂O₂ at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of vWF on cell surface by 89 ± 5% (p < 0.01). Analysis of immunofluorescent images of HUVEC with CellProfiler program revealed that the average number of antigen positive structures on the single cell surface increases from 11.4 ± 0.16 in control up to 17.5 ± 0.21 after incubation with H₂O₂ (p < 0.01). vWF is exposed on the cell surface as dots with the average sizes around 1–3 μm. H₂O₂ causes an increase in the number of these dots and the appearence of the strings of vWF which are absent in control HUVEC. It is suggested that H₂O₂ may serve as a messenger which stimulates vWF exocytosis.

Original language	English
Pages (from-to)	531-537
Number of pages	7
Journal	Biology Bulletin
Volume	44
Issue number	5
DOIs	https://doi.org/10.1134/S106235901705003X
State	Published - 1 Sep 2017
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

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title = "Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells",

abstract = "The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H2O2 at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of vWF on cell surface by 89 ± 5% (p < 0.01). Analysis of immunofluorescent images of HUVEC with CellProfiler program revealed that the average number of antigen positive structures on the single cell surface increases from 11.4 ± 0.16 in control up to 17.5 ± 0.21 after incubation with H2O2 (p < 0.01). vWF is exposed on the cell surface as dots with the average sizes around 1–3 μm. H2O2 causes an increase in the number of these dots and the appearence of the strings of vWF which are absent in control HUVEC. It is suggested that H2O2 may serve as a messenger which stimulates vWF exocytosis.",

author = "Avdonin, {P. V.} and Tsitrina, {A. A.} and Mironova, {G. Y.} and Avdonin, {P. P.} and Zharkikh, {I. L.} and Nadeev, {A. D.} and Goncharov, {N. V.}",

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year = "2017",

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doi = "10.1134/S106235901705003X",

language = "English",

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T1 - Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells

AU - Avdonin, P. V.

AU - Tsitrina, A. A.

AU - Mironova, G. Y.

AU - Avdonin, P. P.

AU - Zharkikh, I. L.

AU - Nadeev, A. D.

AU - Goncharov, N. V.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H2O2 at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of vWF on cell surface by 89 ± 5% (p < 0.01). Analysis of immunofluorescent images of HUVEC with CellProfiler program revealed that the average number of antigen positive structures on the single cell surface increases from 11.4 ± 0.16 in control up to 17.5 ± 0.21 after incubation with H2O2 (p < 0.01). vWF is exposed on the cell surface as dots with the average sizes around 1–3 μm. H2O2 causes an increase in the number of these dots and the appearence of the strings of vWF which are absent in control HUVEC. It is suggested that H2O2 may serve as a messenger which stimulates vWF exocytosis.

AB - The aim of our research was to study the influence of hydrogen peroxide on the exocytosis of von Willebrand factor (vWF) in human umbilical vein endothelial cells (HUVEC). We have found that H2O2 at a non-toxic concentration (100 μM) increases the amount of vWF secreted by HUVEC by 43 ± 14% over control (p < 0.03) and elevates total exposition of vWF on cell surface by 89 ± 5% (p < 0.01). Analysis of immunofluorescent images of HUVEC with CellProfiler program revealed that the average number of antigen positive structures on the single cell surface increases from 11.4 ± 0.16 in control up to 17.5 ± 0.21 after incubation with H2O2 (p < 0.01). vWF is exposed on the cell surface as dots with the average sizes around 1–3 μm. H2O2 causes an increase in the number of these dots and the appearence of the strings of vWF which are absent in control HUVEC. It is suggested that H2O2 may serve as a messenger which stimulates vWF exocytosis.

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SP - 531

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JO - Biology Bulletin

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Hydrogen peroxide stimulates exocytosis of von Willebrand factor in human umbilical vein endothelial cells

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