Hypersensitivity response has negligible impact on Hematopoietic Stem Cells

Nir Bujanover, Roshina Thapa, Oron Goldstein, Leonid Olender, Omri Sharabi, Michael D. Milsom, Roi Gazit

Research output: Contribution to journalArticlepeer-review

Abstract

Immune cells are generated from hematopoietic stem cells (HSCs) in the bone marrow (BM). Immune stimulation can rapidly activate HSCs out of their quiescent state to accelerate the generation of immune cells. HSCs’ activation follows various viral or bacterial stimuli, and we sought to investigate the hypersensitivity immune response. Surprisingly, the Ova-induced hypersensitivity peritonitis model finds no significant changes in BM HSCs. HSC markers cKIT, SCA1, CD48, CD150, and the Fgd5-mCherry reporter showed no significant difference from control. Functionally, hypersensitivity did not alter HSCs' potency, as assayed by transplantation. We further characterized the possible impact of hypersensitivity using RNA-sequencing of HSCs, finding minor changes at the transcriptome level. Moreover, hypersensitivity induced no significant change in the proliferative state of HSCs. Therefore, this study suggests that, in contrast to other immune stimuli, hypersensitivity has no impact on HSCs.

Original languageEnglish
Pages (from-to)1884-1893
Number of pages10
JournalStem Cell Reports
Volume16
Issue number8
DOIs
StatePublished - 10 Aug 2021

Keywords

  • dormancy
  • hematopoietic stem cells
  • hypersensitivity
  • immune response
  • quiescence

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Hypersensitivity response has negligible impact on Hematopoietic Stem Cells'. Together they form a unique fingerprint.

Cite this