TY - JOUR
T1 - Identification and characterization of androgenic gland specific insulin-like peptide-encoding transcripts in two spiny lobster species
T2 - Sagmariasus verreauxi and Jasus edwardsii
AU - Ventura, Tomer
AU - Fitzgibbon, Quinn
AU - Battaglene, Stephen
AU - Sagi, Amir
AU - Elizur, Abigail
N1 - Funding Information:
We thank Karl van Drunen and Melanie Evan for the maintenance of lobsters. This study was supported in part by a Discovery Early Career Research Award granted to Tomer Ventura (DECRA, Grant No. DE130101089 ) by the Australian Research Council (ARC, http://www.arc.gov.au/ ) and an ARC Industrial Transformation Research Hub grant (ITRH, Grant No. IH120100032 ) to Stephen Battaglene and Quinn Fitzgibbon.
Publisher Copyright:
© 2014.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - In this study we describe, for the first time in spiny lobsters, the androgenic gland and its putative hormone. The androgenic gland in crustaceans is the key regulator of crustacean masculinity. The transcript encoding the insulin-like androgenic gland specific factor has recently been identified and characterized in a number of decapod crustacean species including commercially important crabs, crayfish, prawns and shrimps. This insulin-like factor has proven to be the androgenic gland masculinizing hormone, and is absent in females. While the androgenic gland and its putative hormone have been identified in all other commercially valuable groups, none had been identified in lobsters. We identified and characterized the androgenic glands of two spiny lobster species (Sagmariasus verreauxi and Jasus edwardsii) and conducted a transcriptomic analysis of the S. verreauxi androgenic gland. Bioinformatics analysis led to the discovery and characterization of the insulin-like androgenic gland specific factors in both species studied. Changes in androgenic gland cell size and quantity between sub-adult and sexually mature males were evident. The transcriptomic database established for the S. verreauxi androgenic gland might enable to elucidate the mechanisms through which the insulin-like factor is secreted, transported to the target cells and how it triggers the physiological effects of sexual differentiation towards maleness and maintenance of the male gonad.
AB - In this study we describe, for the first time in spiny lobsters, the androgenic gland and its putative hormone. The androgenic gland in crustaceans is the key regulator of crustacean masculinity. The transcript encoding the insulin-like androgenic gland specific factor has recently been identified and characterized in a number of decapod crustacean species including commercially important crabs, crayfish, prawns and shrimps. This insulin-like factor has proven to be the androgenic gland masculinizing hormone, and is absent in females. While the androgenic gland and its putative hormone have been identified in all other commercially valuable groups, none had been identified in lobsters. We identified and characterized the androgenic glands of two spiny lobster species (Sagmariasus verreauxi and Jasus edwardsii) and conducted a transcriptomic analysis of the S. verreauxi androgenic gland. Bioinformatics analysis led to the discovery and characterization of the insulin-like androgenic gland specific factors in both species studied. Changes in androgenic gland cell size and quantity between sub-adult and sexually mature males were evident. The transcriptomic database established for the S. verreauxi androgenic gland might enable to elucidate the mechanisms through which the insulin-like factor is secreted, transported to the target cells and how it triggers the physiological effects of sexual differentiation towards maleness and maintenance of the male gonad.
KW - Androgenic gland
KW - Insulin-like androgenic gland hormone
KW - Jasus edwardsii
KW - Sagmariasus verreauxi
KW - Spiny lobster
UR - http://www.scopus.com/inward/record.url?scp=84928946894&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2014.06.027
DO - 10.1016/j.ygcen.2014.06.027
M3 - Article
AN - SCOPUS:84928946894
SN - 0016-6480
VL - 214
SP - 126
EP - 133
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
ER -