Identification of receptor-interacting regions of vitellogenin within evolutionarily conserved β-sheet structures by using a peptide array

Ziv Roth, Simy Weil, Eliahu D. Aflalo, Rivka Manor, Amir Sagi, Isam Khalaila

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Vitellogenesis, a key process in oviparous animals, is characterized by enhanced synthesis of the lipoprotein vitellogenin, which serves as the major yolk-protein precursor. In most oviparous animals, and specifically in crustaceans, vitellogenin is mainly synthesized in the hepatopancreas, secreted to the hemolymph, and taken up into the ovary by receptor-mediated endocytosis. In the present study, localization of the vitellogenin receptor and its interaction with vitellogenin were investigated in the freshwater prawn Macrobrachium rosenbergii. The receptor was immuno-histochemically localized to the cell periphery and around yolk vesicles. A receptor blot assay revealed that the vitellogenin receptor interacts with most known vitellogenin subunits, the most prominent being the 79 kDa subunit. The receptor was, moreover, able to interact with trypsin-digested vitellogenin peptides. By combining a novel peptide-array approach with tandem mass spectrometry, eleven vitellogenin-derived peptides that interacted with the receptor were identified. A 3D model of vitellogenin indicated that four of the identified peptides are N-terminally localized. One of the peptides is homologous to the receptor-recognized site of vertebrate vitellogenin, and assumes a conserved β-sheet structure. These findings suggest that this specific β-sheet region in the vitellogenin N-terminal lipoprotein domain is the receptor-interacting site, with the rest of the protein serving to enhance affinity for the receptor. The conservation of the receptor recognition site in invertebrate and vertebrate vitellogenin might have vast implications for oviparous species reproduction, development, immunity, and pest management.

Original languageEnglish
Pages (from-to)1116-1122
Number of pages7
JournalChemBioChem
Volume14
Issue number9
DOIs
StatePublished - 1 Jun 2013

Keywords

  • Invertebrates
  • Ligands
  • Peptides
  • Receptor-mediated endocytosis
  • Receptors

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