Identification of the double genome donor in spontaneous triploid tomato plants by RFLP analysis

Miri Lapidot; Dudy Bar-Zvi; V. Kagan-Zur; Yosef Mizrahi

doi:10.1007/BF00220796

Identification of the double genome donor in spontaneous triploid tomato plants by RFLP analysis

Miri Lapidot, Dudy Bar-Zvi, V. Kagan-Zur, Yosef Mizrahi

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Independent spontaneous triploid tomato plants (Lycopersicon esculentum Mill.) were collected among diploid hybrids growing in commercial greenhouses. Ploidy levels were verified by counting chromosomes, and the donor of the double genome dose was determined by restriction fragment length polymorphism (RFLP) analysis. The TG101 probe, which is tightly linked to the Tm-2^a locus, revealed different restriction patterns between TMV-resistant and TMV-susceptible parent lines. The parent donor which provided two genomes to the triploid was identified by comparing the relative intensity of alleles in the triploid with that in the diploid. The results indicate that both parents can serve as a double genome donor.

Original language	English
Pages (from-to)	914-916
Journal	Theoretical And Applied Genetics
Volume	88
Issue number	8
DOIs	https://doi.org/10.1007/BF00220796
State	Published - 1 Sep 1994

Keywords

Lycopersicon esculentum Mill.
Triploid Restriction fragment length polymorphism (RFLP)

ASJC Scopus subject areas

Biotechnology
Agronomy and Crop Science
Genetics

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10.1007/BF00220796

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title = "Identification of the double genome donor in spontaneous triploid tomato plants by RFLP analysis",

abstract = "Independent spontaneous triploid tomato plants (Lycopersicon esculentum Mill.) were collected among diploid hybrids growing in commercial greenhouses. Ploidy levels were verified by counting chromosomes, and the donor of the double genome dose was determined by restriction fragment length polymorphism (RFLP) analysis. The TG101 probe, which is tightly linked to the Tm-2a locus, revealed different restriction patterns between TMV-resistant and TMV-susceptible parent lines. The parent donor which provided two genomes to the triploid was identified by comparing the relative intensity of alleles in the triploid with that in the diploid. The results indicate that both parents can serve as a double genome donor.",

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AU - Lapidot, Miri

AU - Bar-Zvi, Dudy

AU - Kagan-Zur, V.

AU - Mizrahi, Yosef

PY - 1994/9/1

Y1 - 1994/9/1

N2 - Independent spontaneous triploid tomato plants (Lycopersicon esculentum Mill.) were collected among diploid hybrids growing in commercial greenhouses. Ploidy levels were verified by counting chromosomes, and the donor of the double genome dose was determined by restriction fragment length polymorphism (RFLP) analysis. The TG101 probe, which is tightly linked to the Tm-2a locus, revealed different restriction patterns between TMV-resistant and TMV-susceptible parent lines. The parent donor which provided two genomes to the triploid was identified by comparing the relative intensity of alleles in the triploid with that in the diploid. The results indicate that both parents can serve as a double genome donor.

AB - Independent spontaneous triploid tomato plants (Lycopersicon esculentum Mill.) were collected among diploid hybrids growing in commercial greenhouses. Ploidy levels were verified by counting chromosomes, and the donor of the double genome dose was determined by restriction fragment length polymorphism (RFLP) analysis. The TG101 probe, which is tightly linked to the Tm-2a locus, revealed different restriction patterns between TMV-resistant and TMV-susceptible parent lines. The parent donor which provided two genomes to the triploid was identified by comparing the relative intensity of alleles in the triploid with that in the diploid. The results indicate that both parents can serve as a double genome donor.

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Identification of the double genome donor in spontaneous triploid tomato plants by RFLP analysis

Abstract

Keywords

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