Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

S. Nayak; S. K. Singh; N. Ramaiah; R. A. Sreepada

doi:10.1016/j.fsi.2010.05.010

Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

S. Nayak, S. K. Singh, N. Ramaiah, R. A. Sreepada

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi.

Original language	English
Pages (from-to)	544-549
Number of pages	6
Journal	Fish and Shellfish Immunology
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/j.fsi.2010.05.010
State	Published - 1 Jan 2010
Externally published	Yes

Keywords

EST
Penaeus monodon
QPCR
SSH
Shrimp diseases
Vibrio harveyi

ASJC Scopus subject areas

Immunology
Environmental Chemistry
Aquatic Science
Immunology and Microbiology (miscellaneous)

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10.1016/j.fsi.2010.05.010

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title = "Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae",

abstract = "A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi.",

keywords = "EST, Penaeus monodon, QPCR, SSH, Shrimp diseases, Vibrio harveyi",

author = "S. Nayak and Singh, \{S. K.\} and N. Ramaiah and Sreepada, \{R. A.\}",

year = "2010",

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doi = "10.1016/j.fsi.2010.05.010",

language = "English",

volume = "29",

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T1 - Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

AU - Nayak, S.

AU - Singh, S. K.

AU - Ramaiah, N.

AU - Sreepada, R. A.

PY - 2010/1/1

Y1 - 2010/1/1

N2 - A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi.

AB - A subtracted cDNA library was constructed and analyzed to elucidate the response of Penaeus monodon postlarvae challenged with Vibrio harveyi. As many as 960 randomly selected cDNA fragments generated through suppression subtractive hybridization were single pass sequenced. Forty five genes and 20 hypothetical proteins were identified, a few being first reports from shrimps. The most abundant immune relevant genes were ferritin, hemocyanin, and TCTP (translationally controlled tumor protein) indicating their upregulation as also confirmed through qPCR. Post-infection qPCR analyses confirmed 2.04, 2.09, 3.28, 5.49, 6.47, and 11.63 fold rise respectively in ferritin, penaeidin, MnSOD, lysozyme, TCTP, and hemocyanin genes. These genes may be involved in the regulation of the host defense against V. harveyi.

KW - EST

KW - Penaeus monodon

KW - QPCR

KW - SSH

KW - Shrimp diseases

KW - Vibrio harveyi

UR - https://www.scopus.com/pages/publications/77954536931

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DO - 10.1016/j.fsi.2010.05.010

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C2 - 20580834

AN - SCOPUS:77954536931

SN - 1050-4648

VL - 29

SP - 544

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JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 3

ER -

Identification of upregulated immune-related genes in Vibrio harveyi challenged Penaeus monodon postlarvae

Abstract

Keywords

ASJC Scopus subject areas

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