Identification, purification and partial characterization of a 70kDa inhibitor protein of Na+/K+-ATPase from cytosol of pulmonary artery smooth muscle

Kuntal Dey, Tapati Chakraborti, Soumitra Roy, Biswarup Ghosh, Pulak Kar, Sajal Chakraborti

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Aims: We sought to identify, purify and partially characterize a protein inhibitor of Na+/K+-ATPase in cytosol of pulmonary artery smooth muscle. Main methods: (i) By spectrophotometric assay, we identified an inhibitor of Na+/K+-ATPase in cytosolic fraction of pulmonary artery smooth muscle; (ii) the inhibitor was purified by a combination of ammonium sulfate precipitation, diethylaminoethyl (DEAE) cellulose chromatography, hydroxyapatite chromatography and gel filtration chromatography; (iii) additionally, we have also purified Na+/K+-ATPase Α2Β1 and Α1Β1 isozymes for determining some characteristics of the inhibitor. Key findings: We identified a novel endogenous protein inhibitor of Na+/K+-ATPase having an apparent mol mass of ~70kDa in the cytosolic fraction of the smooth muscle. The IC50 value of the inhibitor towards the enzyme was determined to be in the nanomolar range. Important characteristics of the inhibitor are as follows: (i) it showed different affinities toward the ΑΒ2Α1 and Β121 isozymes of the Na+/K+-ATPase; (ii) it interacted reversibly to the E1 site of the enzyme; (iii) the inhibitor blocked the phosphorylated intermediate formation; and (iv) it competitively inhibited the enzyme with respect to ATP. CD studies indicated that the inhibitor causes an alteration of the conformation of the enzyme. The inhibition study also suggested that the DHPC solubilized Na+/K+-ATPase exists as (ΑΒ)2 diprotomer. Significance: The inhibitor binds to the Na+/K+-ATPase at a site different from the ouabain binding site. The novelty of the inhibitor is that it acts in an isoform specific manner on the enzyme, where Α2 is more sensitive than α1.

Original languageEnglish
Pages (from-to)473-481
Number of pages9
JournalLife Sciences
Volume86
Issue number13-14
DOIs
StatePublished - 3 Mar 2010
Externally publishedYes

Keywords

  • Isozyme
  • Na/K-ATPase
  • Na/K-ATPase inhibitor
  • Ouabain

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Pharmacology, Toxicology and Pharmaceutics (all)

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