Identification, purification and partial characterization of low molecular weight protein inhibitor of Na+/K+-ATPase from pulmonary artery smooth muscle cells

Sayed Modinur Rahaman, Kuntal Dey, Partha Das, Soumitra Roy, Tapati Chakraborti, Sajal Chakraborti

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have identified a novel endogenous low mol wt. (15.6 kDa) protein inhibitor of Na+/K+-ATPase in cytosolic fraction of bovine pulmonary artery smooth muscle cells. The inhibitor showed different affinities toward the α2β1 and α1β 1 isozymes of Na+/K+-ATPase, where α2 is more sensitive than α1. The inhibitor interacted reversibly to the E1 site of the enzyme and blocked the phosphorylated intermediate formation. Circular dichroism study suggests that the inhibitor causes an alteration in the confirmation of the enzyme.

Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume393
Issue number1-2
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Ouabain
  • Protein inhibitor
  • Pulmonary smooth muscle cells

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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