Image formation in fluorescence coherence-gated imaging through scattering media

A. Bilenca, T. Lasser, A. Ozcan, R. A. Leitgeb, B. E. Bouma, G. J. Tearney

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Recently, we have experimentally demonstrated a new form of cross-sectional, coherence-gated fluorescence imaging referred to as SD-FCT ('spectral-domain fluorescence coherence tomography'). Imaging in SD-FCT is accomplished by spectrally detecting self-interference of the spontaneous emission of fluorophores, thereby providing depth-resolved information on the axial positions of fluorescent probes. Here, we present a theoretical investigation of the factors affecting the detected SD-FCT signal through scattering media. An imaging equation for SD-FCT is derived that includes the effects of defocusing, numerical-aperture, and the optical properties of the medium. A comparison between the optical sectioning capabilities of SD-FCT and confocal microscopy is also presented. Our results suggest that coherence gating in fluorescence imaging may provide an improved approach for depth-resolved imaging of fluorescently labeled samples; high axial resolution (a few microns) can be achieved with low numerical apertures (N4<0.09) while maintaining a large depth of field (a few hundreds of microns) in a relatively low scattering medium (6 mean free paths), whereas moderate NA's can be used to enhance depth selectivity in more highly scattering biological samples.

Original languageEnglish
Pages (from-to)2810-2821
Number of pages12
JournalOptics Express
Volume15
Issue number6
DOIs
StatePublished - 19 Mar 2007
Externally publishedYes

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