Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval

Shiori Matsuda; Naru Yoneda; Manoj Kumar; Xiangyu Quan; Wataru Watanabe; Osamu Matoba

doi:10.1117/12.3007915

Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval

Shiori Matsuda, Naru Yoneda, Manoj Kumar, Xiangyu Quan, Wataru Watanabe, Osamu Matoba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

A degraded fluorescence image passing through a weak diffuser is improved by the acquisition of fluorescence wavefront information and phase retrieval. The amplitude and phase distributions of the fluorescent light wave are obtained using the transport of intensity equation. After calculating back-propagation to the diffuser surface from the recording plane, phase retrieval is applied for improving the image quality. Experiments using fluorescent beads examined at different recording plane showed the effectiveness of the proposed method.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference
Editors	Toyohiko Yatagai
Publisher	SPIE
ISBN (Electronic)	9781510663435
DOIs	https://doi.org/10.1117/12.3007915
State	Published - 1 Jan 2023
Externally published	Yes
Event	Biomedical Imaging and Sensing Conference 2023 - Yokohama, Japan Duration: 17 Apr 2023 → 21 Apr 2023

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12608
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biomedical Imaging and Sensing Conference 2023
Country/Territory	Japan
City	Yokohama
Period	17/04/23 → 21/04/23

Keywords

fluorescent imaging
imaging through scattering media
transport of intensity equation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.3007915

Cite this

Matsuda, S., Yoneda, N., Kumar, M., Quan, X., Watanabe, W., & Matoba, O. (2023). Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval. In T. Yatagai (Ed.), Biomedical Imaging and Sensing Conference Article 126080V (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608). SPIE. https://doi.org/10.1117/12.3007915

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title = "Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval",

abstract = "A degraded fluorescence image passing through a weak diffuser is improved by the acquisition of fluorescence wavefront information and phase retrieval. The amplitude and phase distributions of the fluorescent light wave are obtained using the transport of intensity equation. After calculating back-propagation to the diffuser surface from the recording plane, phase retrieval is applied for improving the image quality. Experiments using fluorescent beads examined at different recording plane showed the effectiveness of the proposed method.",

keywords = "fluorescent imaging, imaging through scattering media, transport of intensity equation",

author = "Shiori Matsuda and Naru Yoneda and Manoj Kumar and Xiangyu Quan and Wataru Watanabe and Osamu Matoba",

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doi = "10.1117/12.3007915",

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Matsuda, S, Yoneda, N, Kumar, M, Quan, X, Watanabe, W & Matoba, O 2023, Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval. in T Yatagai (ed.), Biomedical Imaging and Sensing Conference., 126080V, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12608, SPIE, Biomedical Imaging and Sensing Conference 2023, Yokohama, Japan, 17/04/23. https://doi.org/10.1117/12.3007915

Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval. / Matsuda, Shiori; Yoneda, Naru; Kumar, Manoj et al.
Biomedical Imaging and Sensing Conference. ed. / Toyohiko Yatagai. SPIE, 2023. 126080V (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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T1 - Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval

AU - Matsuda, Shiori

AU - Yoneda, Naru

AU - Kumar, Manoj

AU - Quan, Xiangyu

AU - Watanabe, Wataru

AU - Matoba, Osamu

PY - 2023/1/1

Y1 - 2023/1/1

N2 - A degraded fluorescence image passing through a weak diffuser is improved by the acquisition of fluorescence wavefront information and phase retrieval. The amplitude and phase distributions of the fluorescent light wave are obtained using the transport of intensity equation. After calculating back-propagation to the diffuser surface from the recording plane, phase retrieval is applied for improving the image quality. Experiments using fluorescent beads examined at different recording plane showed the effectiveness of the proposed method.

AB - A degraded fluorescence image passing through a weak diffuser is improved by the acquisition of fluorescence wavefront information and phase retrieval. The amplitude and phase distributions of the fluorescent light wave are obtained using the transport of intensity equation. After calculating back-propagation to the diffuser surface from the recording plane, phase retrieval is applied for improving the image quality. Experiments using fluorescent beads examined at different recording plane showed the effectiveness of the proposed method.

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KW - imaging through scattering media

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M3 - Conference contribution

AN - SCOPUS:85176122712

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Imaging and Sensing Conference

A2 - Yatagai, Toyohiko

PB - SPIE

T2 - Biomedical Imaging and Sensing Conference 2023

Y2 - 17 April 2023 through 21 April 2023

ER -

Image Recovery of Fluorescent Beads by TIE-based Computational Imaging and Phase Retrieval

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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