TY - JOUR
T1 - Imaging protein-protein interactions in plant cells by bimolecular fluorescence complementation assay
AU - Weinthal, Dan
AU - Tzfira, Tzvi
N1 - Funding Information:
The work in our laboratories is supported by grants from the Consortium for Plant Biotechnology Research (CPBR), the Biotechnology Research and Development Corporation (BRDC) and the National Institutes of Health (NIH). We apologize to those whose work was not cited owing to space constraints.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - The bimolecular fluorescence complementation (BiFC) assay is based on the reconstruction of a fluorescent signal upon the interaction of two protein partners fused to two non-fluorescent fragments of an otherwise fluorescent protein. Interacting partners are typically tagged to fragments of the yellow fluorescent protein, but the use of other fluorescent proteins has been reported. By combining fragments of different types of fluorescent proteins, it is possible not only to detect pairwise protein-protein interaction but also to study the formation of multiprotein complexes in living cells. As we discuss here, a multicolor BiFC set of vectors has been recently deployed for visualizing the simultaneous formation of alternative protein kinase and calcium sensor complexes in living plant cells. This proof-of-concept report and the vectors that have been developed are an important addition to the sets of tools that are useful for analysing multiprotein complexes in plant cells.
AB - The bimolecular fluorescence complementation (BiFC) assay is based on the reconstruction of a fluorescent signal upon the interaction of two protein partners fused to two non-fluorescent fragments of an otherwise fluorescent protein. Interacting partners are typically tagged to fragments of the yellow fluorescent protein, but the use of other fluorescent proteins has been reported. By combining fragments of different types of fluorescent proteins, it is possible not only to detect pairwise protein-protein interaction but also to study the formation of multiprotein complexes in living cells. As we discuss here, a multicolor BiFC set of vectors has been recently deployed for visualizing the simultaneous formation of alternative protein kinase and calcium sensor complexes in living plant cells. This proof-of-concept report and the vectors that have been developed are an important addition to the sets of tools that are useful for analysing multiprotein complexes in plant cells.
UR - http://www.scopus.com/inward/record.url?scp=59349120472&partnerID=8YFLogxK
U2 - 10.1016/j.tplants.2008.11.002
DO - 10.1016/j.tplants.2008.11.002
M3 - Short survey
C2 - 19150604
AN - SCOPUS:59349120472
SN - 1360-1385
VL - 14
SP - 59
EP - 63
JO - Trends in Plant Science
JF - Trends in Plant Science
IS - 2
ER -