Imaging Pseudomonas aeruginosa Biofilm Extracellular Polymer Scaffolds with Amphiphilic Carbon Dots

Margarita Ritenberg, Sukhendu Nandi, Sofiya Kolusheva, Rambabu Dandela, Michael M. Meijler, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Biofilm formation is a critical facet of pathogenesis and resilience of human, animal, and plant bacteria. Extracellular polymeric substances (EPS) constitute the physical scaffolding for bacterial biofilms and thus play central roles in their development and virulence. We show that newly synthesized amphiphilic fluorescent carbon dots (C-dots) readily bind to the EPS scaffold of Pseudomonas aeruginosa, a major biofilm-forming pathogen, resulting in unprecedented microscopic visualization of the EPS structural features. Fluorescence microscopy analysis utilizing the C-dots reveals that the P. aeruginosa EPS matrix exhibits a remarkable dendritic morphology. The experiments further illuminate the growth kinetics of the EPS and the effect of external factors such as temperature. We also show that the amphiphilic C-dot platform enabled screening of substances disrupting biofilm development, specifically quorum sensing inhibitors.

Original languageEnglish
Pages (from-to)1265-1270
Number of pages6
JournalACS Chemical Biology
Volume11
Issue number5
DOIs
StatePublished - 20 May 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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