TY - GEN
T1 - Immobilization of RNA
T2 - IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
AU - Akabayov, B.
AU - Henn, A.
AU - Nautrup-Pedersen, G.
AU - Elbaum, M.
AU - Sagi, I.
N1 - Publisher Copyright:
© 2002 IEEE.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - RNA molecules play important roles in many biological processes including the storage and transfer of information in the cell. Importantly, RNA folds associated with biological functions. The development of new single-molecule methodologies allows to study native RNA molecules, independent in their sizes, in realtime. This requires the immobilization of RNA molecules on a surface. At the present time, however, there is insufficient knowledge on how to optimize the attachment of these molecules to a plane. Here we report a direct approach to immobilize long RNA on a glass surface. Importantly, these procedures can be applied to both native and synthetic RNA molecules to be probed by various single molecule methodologies.
AB - RNA molecules play important roles in many biological processes including the storage and transfer of information in the cell. Importantly, RNA folds associated with biological functions. The development of new single-molecule methodologies allows to study native RNA molecules, independent in their sizes, in realtime. This requires the immobilization of RNA molecules on a surface. At the present time, however, there is insufficient knowledge on how to optimize the attachment of these molecules to a plane. Here we report a direct approach to immobilize long RNA on a glass surface. Importantly, these procedures can be applied to both native and synthetic RNA molecules to be probed by various single molecule methodologies.
KW - Immobilization
KW - RNA
KW - Single molecule
UR - http://www.scopus.com/inward/record.url?scp=84949908126&partnerID=8YFLogxK
U2 - 10.1109/MCTE.2002.1175009
DO - 10.1109/MCTE.2002.1175009
M3 - Conference contribution
AN - SCOPUS:84949908126
T3 - Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
SP - 71
EP - 72
BT - Proceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
PB - Institute of Electrical and Electronics Engineers
Y2 - 6 June 2002 through 9 June 2002
ER -