Immobilization of RNA: Application to single molecule spectroscopy

B. Akabayov, A. Henn, G. Nautrup-Pedersen, M. Elbaum, I. Sagi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

RNA molecules play important roles in many biological processes including the storage and transfer of information in the cell. Importantly, RNA folds associated with biological functions. The development of new single-molecule methodologies allows to study native RNA molecules, independent in their sizes, in realtime. This requires the immobilization of RNA molecules on a surface. At the present time, however, there is insufficient knowledge on how to optimize the attachment of these molecules to a plane. Here we report a direct approach to immobilize long RNA on a glass surface. Importantly, these procedures can be applied to both native and synthetic RNA molecules to be probed by various single molecule methodologies.

Original languageEnglish
Title of host publicationProceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
PublisherInstitute of Electrical and Electronics Engineers
Pages71-72
Number of pages2
ISBN (Electronic)0780375572, 9780780375574
DOIs
StatePublished - 1 Jan 2002
Externally publishedYes
EventIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002 - Genoa, Italy
Duration: 6 Jun 20029 Jun 2002

Publication series

NameProceedings of the IEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002

Conference

ConferenceIEEE-EMBS Special Topic Conference on Molecular, Cellular and Tissue Engineering, MCTE 2002
Country/TerritoryItaly
CityGenoa
Period6/06/029/06/02

Keywords

  • Immobilization
  • RNA
  • Single molecule

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Biotechnology

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