TY - JOUR
T1 - Immobilization strategies of Brucella particles on optical fibers for use in chemiluminescence immunosensors
AU - Liebes, Yael
AU - Amir, Liron
AU - Marks, Robert S.
AU - Banai, Menachem
N1 - Funding Information:
Dr. Gil Korner, Kamada, Israel, is kindly thanked for his work in purifying the anti-B. melitensis IgG bovine antibodies used in this research. Luba Burlaka from the Center for Meso, Nanoscale Science and Technology, Department of Chemical engineering (BGU), is thanked for performing SEM-EDS measurements and Inbal Tsarfati and Dr. Levi Gheber, Department of Biotechnology Engineering (BGU) are thanked for performing the AFM measurements. Gina Hughes is thanked for her editorial help and Dr. Michal Shani-Sekler and Dr. Sebastien Herrmann are thanked for their advice and insights. This work was supported by Yeshaya Horowitz Association, project BioPen #84534101 and the Technical Support Working Group project #T-582.
PY - 2009/11/15
Y1 - 2009/11/15
N2 - Immunosensors are powerful analytical tools in clinical and veterinary diagnostics. This has led us to design a chemiluminescent immunosensor aimed at identifying anti-Brucella antibodies using optical fibers as the transducer. In order to develop the optimal transducer, to achieve an optimal chemical modification thereby allowing an optimal covalent binding of the protein receptor, several cleaning strategies and silane coupling agents were investigated. Brucella killed organisms were used as a model receptor for quantifying anti-Brucella IgG antibodies in a suspension compared to conventional colorimetric and chemiluminescent ELISA. A silane-benzophenone derivative was selected as the best performing silane coupling agent: the optical fiber immunosensor (OFIS) has showed the lowest limit of detection at 0.207 μg/ml, compared to 0.828 μg/ml and 0.414 μg/ml achieved by colorimetric and chemiluminescent ELISAs, respectively. These results, together with the additional advantages of rapidity, lower reagent volumes and moderate operating conditions, have set the grounds for further study in order to adapt this platform for on-site diagnostics of brucellosis disease markers.
AB - Immunosensors are powerful analytical tools in clinical and veterinary diagnostics. This has led us to design a chemiluminescent immunosensor aimed at identifying anti-Brucella antibodies using optical fibers as the transducer. In order to develop the optimal transducer, to achieve an optimal chemical modification thereby allowing an optimal covalent binding of the protein receptor, several cleaning strategies and silane coupling agents were investigated. Brucella killed organisms were used as a model receptor for quantifying anti-Brucella IgG antibodies in a suspension compared to conventional colorimetric and chemiluminescent ELISA. A silane-benzophenone derivative was selected as the best performing silane coupling agent: the optical fiber immunosensor (OFIS) has showed the lowest limit of detection at 0.207 μg/ml, compared to 0.828 μg/ml and 0.414 μg/ml achieved by colorimetric and chemiluminescent ELISAs, respectively. These results, together with the additional advantages of rapidity, lower reagent volumes and moderate operating conditions, have set the grounds for further study in order to adapt this platform for on-site diagnostics of brucellosis disease markers.
KW - Brucellosis
KW - Chemiluminescence (CL)
KW - Enzyme-linked immuno-sorbent assay (ELISA)
KW - Immunosensor
KW - Optical fiber immunosensor (OFIS)
KW - Silane coupling agent (SCA)
UR - https://www.scopus.com/pages/publications/70349241545
U2 - 10.1016/j.talanta.2009.06.070
DO - 10.1016/j.talanta.2009.06.070
M3 - Article
AN - SCOPUS:70349241545
SN - 0039-9140
VL - 80
SP - 338
EP - 345
JO - Talanta
JF - Talanta
IS - 1
ER -