Immune response to a syngeneic mammary adenocarcinoma. II. In vitro generation of cytotoxic lymphocytes

O. Kuperman, G. W. Fortner, Z. J. Lucas

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A method is described for the consistent in vitro generation of cytotoxic cells by incubating Fischer 344 rat spleen cells on monolayers of a syngeneic mammary adenocarcinoma. Significant cytotoxicity by in vitro culture is generated as early as 3 days after initiation and effector cells are cytolytic only toward target cells of the sensitizing monolayer. Reciprocal sensitization with allogenic fibroblasts as the immunizing monolayer yielded effector cells cytolytic for the fibroblasts but without effect on the mammary tumor. The consistency in the generation of cytotoxic cells by in vitro culture should permit its standardized use in following other related immune phenomena such as blocking by serologic factors and suppression, recruitment or memory for cytotoxic function.

Original languageEnglish
Pages (from-to)1277-1281
Number of pages5
JournalJournal of Immunology
Volume115
Issue number5
StatePublished - 1 Dec 1975
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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