Immunosufficiency can be evaluated by Ig secretion subsequent to mitogenic stimulation of human mononuclear cells (MNC). It seems that there are significant differences in immunoglobulin class secreted by these cells when stimulated with various polyclonal activators. The aim of the current study was to analyse these differences. MNC cells was randomly obtained from nine healthy blood donors and were activated by Epstein-Barr virus (EBV), group-A streptococcus (A-ScM), Staphylococcus aureus (SAC), Klebsiella pneumonia (Kleb-M) and pokeweed mitogen (PWM). Significantly increased levels of IgM were recorded after a 7 day incubation followed by stimulation with Kleb-M (6.2 ± 2.9) and EBV (5.9 ± 4.5) compared to inactivated MNC (1.6 ± 1.4), and following 10 days incubation then stimulation by EBV (13.4 ± 5.5) and Kleb-M (9.9 ± 4.2) compared to unstimulated cells (2.9 ± 1.8). Significantly greater IgG levels were achieved following incubation with EBV (3.0 ± 4.0) and PWM (2.4 ± 1.3) after 7 days (vs 0.6 ± 0.4 in unstimulated cells) and by PWM (11.7 ± 5.3) and Kleb-M 416 vs (2.3 ± 2.2) after 10 days. The present data emphasize the significance of merging both mitogen selection and culture duration for acquiring information and high fidelity results of immunoglobulin secretion by polyclonal activators.
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