Immunoperoxidase assay for detection of specific IgG and IgA antibodies to human spermatozoa in infertile women

G. Holcberg, D. Kleinman, I. Sarov, G. Potashnik, V. Insler

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    A new immunoperoxidase antibody-membrane antigen (IPAMA) technique for the detection of IgG and IgA antibodies specific for sperm antigens is described. The technique utilizes as antigen sperm cells dried on glass slides and stored at -70°C. Sera of 82 infertile women, 50 primiparas, 50 multiparas and 25 children were tested for IPAMA. Results obtained with the IPAMA test for sperm-specific IgG are compared with the results obtained by the sperm immobilization test (SIT). By the IPAMA technique, 11 of 82 infertile women (13.4%) were positive for sperm-specific IgG antibodies, 12 (14.6%) for IgA antibodies and three (3.7%) for both. In the control groups only two of 125 subjects were positive for sperm-specific IgG and none for IgA antibodies. The difference in the prevalence of anti-sperm antibodies between the control groups and the infertility group was highly significant (P < 0.001). There was agreement between the results of the IPAMA and SIT as to the presence or absence of antibodies in seven of the 11 IgG-positive sera in the infertility group. Four more sera were IgG positive by IPAMA only. The two positive sera in the control group were detected by both IPAMA and SIT. Since the IPAMA technique is simple, does not require special equipment and utilizes stored antigen, it seems that this method could be useful in mass screening of infertile couples.

    Original languageEnglish
    Pages (from-to)28-39
    Number of pages12
    JournalInternational Journal of Fertility
    Volume31
    Issue number1
    StatePublished - 15 Oct 1986

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

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