Impedance study of the hybrid molecule alginate-pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

Khalil Abu-Rabeah; Robert S. Marks

doi:10.1016/j.snb.2008.09.020

Impedance study of the hybrid molecule alginate-pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

Khalil Abu-Rabeah, Robert S. Marks

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The aim of this paper is to report the characterization and application of a new and attractive hybrid molecule (alginate modified with pyrrole) alginate-pyrrole (Alg-py) with glucose oxidase (GOx) to create a highly responsive glucose biosensor. The biosensor was built by covalent attachment of GOx with the Alg-py matrix, followed by pyrrole polymerization, then casting the biocomposite onto the surface of a platinum electrode (Pt). The unique three-dimensional structure, porous morphology, conductive setup, mechanical firmness and biocompatibility resulted in low response time (4 s), high sensitivity value (16μAmM^-1 cm^-2) and a very low enzyme load (9.2 U).

Original language	English
Pages (from-to)	516-522
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	136
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2008.09.020
State	Published - 1 Jan 2009

Keywords

Alginate-pyrrole
Biosensor
Conductive polymers
Glucose oxidase
Hydrogel

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2008.09.020

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abstract = "The aim of this paper is to report the characterization and application of a new and attractive hybrid molecule (alginate modified with pyrrole) alginate-pyrrole (Alg-py) with glucose oxidase (GOx) to create a highly responsive glucose biosensor. The biosensor was built by covalent attachment of GOx with the Alg-py matrix, followed by pyrrole polymerization, then casting the biocomposite onto the surface of a platinum electrode (Pt). The unique three-dimensional structure, porous morphology, conductive setup, mechanical firmness and biocompatibility resulted in low response time (4 s), high sensitivity value (16μAmM-1 cm-2) and a very low enzyme load (9.2 U).",

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AB - The aim of this paper is to report the characterization and application of a new and attractive hybrid molecule (alginate modified with pyrrole) alginate-pyrrole (Alg-py) with glucose oxidase (GOx) to create a highly responsive glucose biosensor. The biosensor was built by covalent attachment of GOx with the Alg-py matrix, followed by pyrrole polymerization, then casting the biocomposite onto the surface of a platinum electrode (Pt). The unique three-dimensional structure, porous morphology, conductive setup, mechanical firmness and biocompatibility resulted in low response time (4 s), high sensitivity value (16μAmM-1 cm-2) and a very low enzyme load (9.2 U).

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Impedance study of the hybrid molecule alginate-pyrrole: Demonstration as host matrix for the construction of a highly sensitive amperometric glucose biosensor

Abstract

Keywords

ASJC Scopus subject areas

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