Impedimetric quantification of anti-dengue antibodies using functional carbon nanotube deposits validated with blood plasma assays

Quentin Palomar, Chantal Gondran, Robert Marks, Serge Cosnier, Michael Holzinger

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A high performance impedimetric immunosensor for the dengue virus antibody detection is presented. The setup profits from the formation of controlled and reproducible carbon nanotube (CNT) deposits on electrodes. Their easy functionalization via electrogeneration of a polypyrrole-NHS (N-hydroxysuccinimido 11-(pyrrol-1-yl) undecanoate) film enables the immobilization of the Dengue Virus 2 NS1 glycoprotein, the receptor unit, on the porous CNT layer via covalent amide coupling to provide the necessary selectivity towards Dengue NS1 antibody. All building steps of this immunosensor and the performance of this system were monitored by impedance spectroscopy and cyclic voltammetry. The resulting impedimetric dengue biosensor was tested in bovine blood plasma in addition to conventional measurements under controlled environment. After optimization, this immunosensor shows a good linearity in a wide concentration range (10−13–10−5 g mL−1).

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalElectrochimica Acta
Volume274
DOIs
StatePublished - 1 Jun 2018

Keywords

  • Carbon nanotube deposits
  • Dengue virus antibody
  • Electrochemical impedance spectroscopy
  • Immunosensor

ASJC Scopus subject areas

  • General Chemical Engineering
  • Electrochemistry

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