Improved enzyme retention from an electropolymerized polypyrrole-alginate matrix in the development of biosensors

Rodica E. Ionescu, Khalil Abu-Rabeah, Serge Cosnier, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A novel pyrrole-alginate was synthesized providing a gel by Ca2+ cross-linking. The subsequent in situ electrochemical polymerization of the linked pyrrole groups at 0.93 V led to the formation of a composite polypyrrole-gel matrix exhibiting a greater enzyme retention as well as increased alginate stability towards the destructive effect of phosphate anions unlike the natural alginate gel. The presence of the electropolymerized chains was clearly indicated by the decrease of the permeability when compared to natural alginate gel, namely 2.7 × 10-2 and 3.65 × 10-1 cm s-1 respectively, using as an electroactive permeate, hydroquinone. Moreover, the analytical performance of glucose oxidase-based composite alginate for the determination of glucose was determined.

Original languageEnglish
Pages (from-to)1277-1282
Number of pages6
JournalElectrochemistry Communications
Volume7
Issue number12
DOIs
StatePublished - 1 Dec 2005

Keywords

  • Alginate
  • Biosensor
  • Electropolymerization
  • Permeability
  • Pyrrole-alginate

ASJC Scopus subject areas

  • Electrochemistry

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