TY - JOUR
T1 - In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration
T2 - Production Parameters and In Vitro Characterization
AU - Kruppke, Benjamin
AU - Farack, Jana
AU - Sommer, Freya
AU - Weil, Simy
AU - Aflalo, Eliahu David
AU - Wiesmann, Hans Peter
AU - Sagi, Amir
AU - Hanke, Thomas
N1 - Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily-, and twice-a-week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate-buffered saline.
AB - Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily-, and twice-a-week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate-buffered saline.
KW - bioactivity
KW - bone substitute material
KW - chitosan
KW - compressive strength
KW - crosslinking
KW - scaffold
UR - http://www.scopus.com/inward/record.url?scp=85022331070&partnerID=8YFLogxK
U2 - 10.1002/mame.201700147
DO - 10.1002/mame.201700147
M3 - Article
AN - SCOPUS:85022331070
SN - 1438-7492
VL - 302
JO - Macromolecular Materials and Engineering
JF - Macromolecular Materials and Engineering
IS - 10
M1 - 1700147
ER -