In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization

Benjamin Kruppke, Jana Farack, Freya Sommer, Simy Weil, Eliahu David Aflalo, Hans Peter Wiesmann, Amir Sagi, Thomas Hanke

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Various methods of chitosan scaffold production are reported in the literature so far. Here, in situ crosslinking with glutaraldehyde is reported for the first time. It combines pore formation and chitosan crosslinking in a single step. This combination allows incorporation of fragile molecules into 3D porous chitosan scaffolds produced by simple and gentle lyophilization. In this study, parameters of in situ crosslinking of porous chitosan scaffold formation as well as their effect on degradation and bioactivity of the scaffolds are examined. The scaffolds are characterized in the context of their prospective application as bone substitute material. The addition of calcium phosphate phases (hydroxyapatite, brushite) to the macroporous chitosan scaffolds allows manipulation of the bioactivity that is investigated by incubation in simulated body fluid (SBF). The bioactivity is significantly influenced by the modus of changing the fluid (static, daily-, and twice-a-week change). Scaffolds are morphologically characterized by means of scanning electron microscopy, and the mechanical stability is tested after incubation in SBF and phosphate-buffered saline.

Original languageEnglish
Article number1700147
JournalMacromolecular Materials and Engineering
Volume302
Issue number10
DOIs
StatePublished - 1 Oct 2017

Keywords

  • bioactivity
  • bone substitute material
  • chitosan
  • compressive strength
  • crosslinking
  • scaffold

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