Abstract
Tobacco protoplasts obtained from leaf mesophyll cells and suspended in agar nutrient medium divided and produced colonies only when plated at high densities, above 104 cells per ml. At such densities coalescence of the expanding colonies occurred at high frequency. Nondividing X-irradiated protoplasts used as feeder cells supported division of viable protoplasts plated at densities as low as 102 cells per ml. The feeder cell technique should thus facilitate the application of screening procedures for the isolation of colonies originating from single mutated cells occurring in a suspended population.
Original language | English |
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Pages (from-to) | 216-222 |
Number of pages | 7 |
Journal | In Vitro |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - 1 Dec 1973 |
Externally published | Yes |
ASJC Scopus subject areas
- Biotechnology
- Plant Science