In vitro culture of tobacco protoplasts: Use of feeder techniques to support division of cells plated at low densities

Dina Raveh, Eliezer Huberman, Esra Galun

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Tobacco protoplasts obtained from leaf mesophyll cells and suspended in agar nutrient medium divided and produced colonies only when plated at high densities, above 104 cells per ml. At such densities coalescence of the expanding colonies occurred at high frequency. Nondividing X-irradiated protoplasts used as feeder cells supported division of viable protoplasts plated at densities as low as 102 cells per ml. The feeder cell technique should thus facilitate the application of screening procedures for the isolation of colonies originating from single mutated cells occurring in a suspended population.

Original languageEnglish
Pages (from-to)216-222
Number of pages7
JournalIn Vitro
Volume9
Issue number3
DOIs
StatePublished - 1 Dec 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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