In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata

H. Kalamarz-Kubiak; I. Meiri-Ashkenazi; A. Kleszczyńska; H. Rosenfeld

doi:10.1111/jfb.12297

In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata

H. Kalamarz-Kubiak, I. Meiri-Ashkenazi, A. Kleszczyńska, H. Rosenfeld

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4×10^-8, 1·4×10^-7 and 0·4×10^-6M cortisol and 10^-12, 10^-10 and 10^-8M UI for 6, 24 and 48h, respectively. AVT and IT contents were determined in the culture media by high-performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6h of incubation at all doses. After 24h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic-pituitary-interrenal (HPI) axis in this species.

Original language	English
Pages (from-to)	448-458
Number of pages	11
Journal	Journal of Fish Biology
Volume	84
Issue number	2
DOIs	https://doi.org/10.1111/jfb.12297
State	Published - 1 Feb 2014
Externally published	Yes

Keywords

Fish nonapeptides
Primary hypophysis cell cultures
Stress hormones

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Aquatic Science

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10.1111/jfb.12297

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title = "In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata",

abstract = "This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4×10-8, 1·4×10-7 and 0·4×10-6M cortisol and 10-12, 10-10 and 10-8M UI for 6, 24 and 48h, respectively. AVT and IT contents were determined in the culture media by high-performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6h of incubation at all doses. After 24h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic-pituitary-interrenal (HPI) axis in this species.",

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AU - Kalamarz-Kubiak, H.

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AU - Kleszczyńska, A.

AU - Rosenfeld, H.

PY - 2014/2/1

Y1 - 2014/2/1

N2 - This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4×10-8, 1·4×10-7 and 0·4×10-6M cortisol and 10-12, 10-10 and 10-8M UI for 6, 24 and 48h, respectively. AVT and IT contents were determined in the culture media by high-performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6h of incubation at all doses. After 24h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic-pituitary-interrenal (HPI) axis in this species.

AB - This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4×10-8, 1·4×10-7 and 0·4×10-6M cortisol and 10-12, 10-10 and 10-8M UI for 6, 24 and 48h, respectively. AVT and IT contents were determined in the culture media by high-performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6h of incubation at all doses. After 24h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic-pituitary-interrenal (HPI) axis in this species.

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In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata

Abstract

Keywords

ASJC Scopus subject areas

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