In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner; Yael Timore; Itzhak Wirguin; Oded Abramsky; Michael Steinitz

doi:10.1016/0165-5728(89)90119-7

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner, Yael Timore, Itzhak Wirguin, Oded Abramsky, Michael Steinitz

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	Journal of Neuroimmunology
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/0165-5728(89)90119-7
State	Published - 1 Jan 1989
Externally published	Yes

Keywords

Anti-acetylcholine receptor antibody
Autoantibody, production in vitro
Epstein-Barr virus-transformed B lymphocyte
Myasthenia gravis

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0165-5728(89)90119-7

Cite this

@article{b109ffcb085d459c98d081b261bc17ec,

title = "In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis",

abstract = "Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.",

keywords = "Anti-acetylcholine receptor antibody, Autoantibody, production in vitro, Epstein-Barr virus-transformed B lymphocyte, Myasthenia gravis",

author = "Talma Brenner and Yael Timore and Itzhak Wirguin and Oded Abramsky and Michael Steinitz",

note = "Funding Information: This work was supported in part by a grant from the Ministry of Trade and Industry and in part by the Reichman Endowment Fund for Research in Neurological Diseases, Israel.",

year = "1989",

month = jan,

day = "1",

doi = "10.1016/0165-5728(89)90119-7",

language = "English",

volume = "24",

pages = "217--222",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

AU - Brenner, Talma

AU - Timore, Yael

AU - Wirguin, Itzhak

AU - Abramsky, Oded

AU - Steinitz, Michael

N1 - Funding Information: This work was supported in part by a grant from the Ministry of Trade and Industry and in part by the Reichman Endowment Fund for Research in Neurological Diseases, Israel.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

AB - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

KW - Anti-acetylcholine receptor antibody

KW - Autoantibody, production in vitro

KW - Epstein-Barr virus-transformed B lymphocyte

KW - Myasthenia gravis

UR - http://www.scopus.com/inward/record.url?scp=0024468527&partnerID=8YFLogxK

U2 - 10.1016/0165-5728(89)90119-7

DO - 10.1016/0165-5728(89)90119-7

M3 - Article

C2 - 2553772

AN - SCOPUS:0024468527

SN - 0165-5728

VL - 24

SP - 217

EP - 222

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 3

ER -

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this