In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner; Yael Timore; Itzhak Wirguin; Oded Abramsky; Michael Steinitz

doi:10.1016/0165-5728(89)90119-7

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Talma Brenner, Yael Timore, Itzhak Wirguin, Oded Abramsky, Michael Steinitz

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	Journal of Neuroimmunology
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/0165-5728(89)90119-7
State	Published - 1 Jan 1989
Externally published	Yes

Keywords

Anti-acetylcholine receptor antibody
Autoantibody, production in vitro
Epstein-Barr virus-transformed B lymphocyte
Myasthenia gravis

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0165-5728(89)90119-7

Cite this

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title = "In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis",

abstract = "Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.",

keywords = "Anti-acetylcholine receptor antibody, Autoantibody, production in vitro, Epstein-Barr virus-transformed B lymphocyte, Myasthenia gravis",

author = "Talma Brenner and Yael Timore and Itzhak Wirguin and Oded Abramsky and Michael Steinitz",

note = "Funding Information: This work was supported in part by a grant from the Ministry of Trade and Industry and in part by the Reichman Endowment Fund for Research in Neurological Diseases, Israel.",

year = "1989",

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doi = "10.1016/0165-5728(89)90119-7",

language = "English",

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T1 - In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

AU - Brenner, Talma

AU - Timore, Yael

AU - Wirguin, Itzhak

AU - Abramsky, Oded

AU - Steinitz, Michael

N1 - Funding Information: This work was supported in part by a grant from the Ministry of Trade and Industry and in part by the Reichman Endowment Fund for Research in Neurological Diseases, Israel.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

AB - Peripheral blood lymphocytes and B-cells were obtained from patients with myasthenia gravis and stimulated in vitro with either pokeweed mitogen or Epstein-Barr virus (EBV), respectively. EBV stimulation of B-cells caused a production of antibodies to acetylcholine receptor in 15 of the 25 myasthenia gravis patients: the EBV stimulation of B-cells was more effective in this regard than the pokeweed mitogen stimulation of peripheral blood lymphocytes. The in vitro synthesis of anti-acetylcholine receptor antibodies was found to be positively correlated with both the patient's sera antibody titers and with the disease severity.

KW - Anti-acetylcholine receptor antibody

KW - Autoantibody, production in vitro

KW - Epstein-Barr virus-transformed B lymphocyte

KW - Myasthenia gravis

UR - https://www.scopus.com/pages/publications/0024468527

U2 - 10.1016/0165-5728(89)90119-7

DO - 10.1016/0165-5728(89)90119-7

M3 - Article

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AN - SCOPUS:0024468527

SN - 0165-5728

VL - 24

SP - 217

EP - 222

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 3

ER -

In vitro synthesis of antibodies to acetylcholine receptor by Epstein-Barr virus-stimulated B-lymphocytes derived from patients with myasthenia gravis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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