In Vivo Biogenesis of a de Novo Designed Iron-Sulfur Protein

Bhanu P. Jagilinki, Stefan Ilic, Cristian Trncik, Alexei M. Tyryshkin, Douglas H. Pike, Wolfgang Lubitz, Eckhard Bill, Oliver Einsle, James A. Birrell, Barak Akabayov, Dror Noy, Vikas Nanda

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In vivo expression of metalloproteins requires specific metal trafficking and incorporation machinery inside the cell. Synthetic designed metalloproteins are typically purified without the target metal, which is subsequently introduced through in vitro reconstitution. The extra step complicates protein optimization by high-throughput library screening or laboratory evolution. We demonstrate that a designed coiled-coil iron-sulfur protein (CCIS) assembles robustly with [4Fe-4S] clusters in vivo. While in vitro reconstitution produces a mixture of oligomers that depends on solution conditions, in vivo production generates a stable homotrimer coordinating a single, diamagnetic [4Fe-4S]2+ cluster. The multinuclear cluster of in vivo assembled CCIS is more resistant to degradation by molecular oxygen. Only one of the two metal coordinating half-sites is required in vivo, indicating specificity of molecular recognition in recruitment of the metal cluster. CCIS, unbiased by evolution, is a unique platform to examine iron-sulfur protein biogenesis and develop synthetic multinuclear oxidoreductases.

Original languageEnglish
Pages (from-to)3400-3407
Number of pages8
JournalACS Synthetic Biology
Volume9
Issue number12
DOIs
StatePublished - 18 Dec 2020

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Fingerprint

Dive into the research topics of 'In Vivo Biogenesis of a de Novo Designed Iron-Sulfur Protein'. Together they form a unique fingerprint.

Cite this