TY - GEN
T1 - In-vivo dehydration sensing in transgenic tobacco plants using an integrated electrochemical chip
AU - Desagani, Dayananda
AU - Jog, Aakash
AU - Avni, Adi
AU - Shacham-Diamand, Yosi
N1 - Funding Information:
This research was supported by the Israel Science Foundation (grant no. 1616/17 titled “Feasibility and Modeling of Integrated Plant Cell Functional sensors”). We would also like to acknowledge the Boris Mints Institute for Strategic Policy Solutions to Global Challenges, the Department of Public Policy, and the Manna Centre for Food Security, Tel Aviv University for their generous support under the program “Plant-based heat stress whole-cell-biosensor” (grant no. 590351, 2017). We also wish to thank the Israeli Ministry of Science, Technology and Space, Infrastructure program, for their generous support under the program “Nanoscale electrodes for single-cell implants” (grant no. 3-14345).
Publisher Copyright:
© 2021 IEEE
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In this paper, we demonstrate in-vivo plant based dehydration sensing using a bio-electrochemical sensor. In-vivo sensing reports on the plant's status as provided by its gene expression, responding to stress. Plant based sensing provides precise, real-time information acquired from the plants themselves; it complements the information provided by ex-vivo sensors sampling the plant's surroundings, e.g. in the soil, or sensors measuring the plant's electrical conductivity. In this paper, we present a method in which the plant's dehydration levels are monitored in real time using in-vivo techniques using the plant as the sensor. In this method, the expression of the β-Dglucuronidase enzyme, expressed under drought conditions, is monitored using its reaction with a substrate, which produces an electrochemically active product. The product is oxidized on the working electrode of a three-electrode electrochemical chip mounted on the leaves of Nicotiana tabacum plants. Electrochemical sensing showed earlier detection compared to other methods, e.g. visual inspection and conductivity measurements.
AB - In this paper, we demonstrate in-vivo plant based dehydration sensing using a bio-electrochemical sensor. In-vivo sensing reports on the plant's status as provided by its gene expression, responding to stress. Plant based sensing provides precise, real-time information acquired from the plants themselves; it complements the information provided by ex-vivo sensors sampling the plant's surroundings, e.g. in the soil, or sensors measuring the plant's electrical conductivity. In this paper, we present a method in which the plant's dehydration levels are monitored in real time using in-vivo techniques using the plant as the sensor. In this method, the expression of the β-Dglucuronidase enzyme, expressed under drought conditions, is monitored using its reaction with a substrate, which produces an electrochemically active product. The product is oxidized on the working electrode of a three-electrode electrochemical chip mounted on the leaves of Nicotiana tabacum plants. Electrochemical sensing showed earlier detection compared to other methods, e.g. visual inspection and conductivity measurements.
KW - Biosensors
KW - Drought plants
KW - Electrochemical sensing
KW - In-vivo plant sensing
UR - http://www.scopus.com/inward/record.url?scp=85098741813&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85098741813
T3 - Proceedings - IEEE International Symposium on Circuits and Systems
BT - 2020 IEEE International Symposium on Circuits and Systems, ISCAS 2020 - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 52nd IEEE International Symposium on Circuits and Systems, ISCAS 2020
Y2 - 10 October 2020 through 21 October 2020
ER -