TY - JOUR
T1 - XIncisive Imaging and Computation for Cellular mysteries
T2 - Lessons from Abscission
AU - Elia, Natalie
AU - Ott, Carolyn
AU - Lippincott-Schwartz, Jennifer
N1 - Funding Information:
The authors thank Erin Fincher and Nicole Jonas from the NICHD Unit on Computer Support Services for creating the graphics used in the figures. We also thank Christian Hellriegel (Zeiss), and members of the Lippincott-Schwartz laboratory, Schuyler VanEngelenberg, Prabuddha Sengupta, Angelika Rambold, and Bennet Waxse for helpful comments on the text. N.E. is supported by the Marie Curie Intergration grant (CIG), EU and by the Israel Science foundadtion (ISF).
PY - 2013/12/5
Y1 - 2013/12/5
N2 - The final cleavage event that terminates cell division, abscission of the small, dense intercellular bridge, has been particularly challenging to resolve. Here, we describe imaging innovations that helped answer long-standing questions about the mechanism of abscission. We further explain how computational modeling of high-resolution data was employed to test hypotheses and generate additional insights. We present the model that emerges from application of these complimentary approaches. Similar experimental strategies will undoubtedly reveal exciting details about other underresolved cellular structures.
AB - The final cleavage event that terminates cell division, abscission of the small, dense intercellular bridge, has been particularly challenging to resolve. Here, we describe imaging innovations that helped answer long-standing questions about the mechanism of abscission. We further explain how computational modeling of high-resolution data was employed to test hypotheses and generate additional insights. We present the model that emerges from application of these complimentary approaches. Similar experimental strategies will undoubtedly reveal exciting details about other underresolved cellular structures.
UR - http://www.scopus.com/inward/record.url?scp=84890093005&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2013.11.011
DO - 10.1016/j.cell.2013.11.011
M3 - Review article
C2 - 24315094
AN - SCOPUS:84890093005
SN - 0092-8674
VL - 155
SP - X1220-1231
JO - Cell
JF - Cell
IS - 6
ER -