TY - JOUR
T1 - Increased plasma and optic nerve levels of IL-6, TNF-α, and MIP-2 following induction of ischemic optic neuropathy in mice
AU - Avraham, Bat Chen R.
AU - Dotan, Gad
AU - Hasanreisoglu, Murat
AU - Kramer, Michal
AU - Monselise, Yehudit
AU - Cohen, Yoram
AU - Weinberger, Dov
AU - Goldenberg-Cohen, Nitza
N1 - Funding Information:
The authors wish to thank Shmuel Behrman for polishing and duplicating the external lenses for the mouse eyes, which were kindly developed and donated by Dr. Steven Brenstein, University of Maryland, Baltimore, Maryland, USA; Dr. Hely Oren-Hazan for her technical support; Vivi Dagan for the graphical editing; Dr. Dorit Karesh for the statistical analysis; and Gloria Ginzach and Hanni Penn for their editorial and secretarial assistance. This study was partially supported by the Zanvyl and Isabelle Krieger Fund, Baltimore, Maryland, USA, and the Tiber Fund, Tel Aviv University, Tel Aviv, Israel (GD, MK).
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Purpose: To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION). Methods: AION was induced in C57/BL6 mice and levels of IL-6, TNF-α, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals. Results: Plasma: IL-6 levels were elevated immediately after rAION induction and decreased gradually thereafter. TNF-α showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-α, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3. Conclusions: Proinflammatory cytokines may play a role in the pathogenesis of rAION.
AB - Purpose: To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION). Methods: AION was induced in C57/BL6 mice and levels of IL-6, TNF-α, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals. Results: Plasma: IL-6 levels were elevated immediately after rAION induction and decreased gradually thereafter. TNF-α showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-α, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3. Conclusions: Proinflammatory cytokines may play a role in the pathogenesis of rAION.
KW - Anterior ischemic optic neuropathy
KW - IL-6
KW - MIP-2
KW - Proinflammatory cytokines
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=41949098556&partnerID=8YFLogxK
U2 - 10.1080/02713680801932891
DO - 10.1080/02713680801932891
M3 - Article
C2 - 18398714
AN - SCOPUS:41949098556
SN - 0271-3683
VL - 33
SP - 395
EP - 401
JO - Current Eye Research
JF - Current Eye Research
IS - 4
ER -