Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity

Ali Nejidat; Genfa Zhang; Michal Grinberg; Yair M. Heimer

doi:10.1016/S0168-9452(97)00203-3

Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity

Ali Nejidat, Genfa Zhang, Michal Grinberg, Yair M. Heimer

Zuckerberg Institute for Water Research

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2-4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	Plant Science
Volume	130
Issue number	1
DOIs	https://doi.org/10.1016/S0168-9452(97)00203-3
State	Published - 5 Dec 1997

Keywords

Arabidopsis thaliana
Chimeric gene
Nitrate
Nitrate reductase
Over- expression
Protein content

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

Access to Document

10.1016/S0168-9452(97)00203-3

Cite this

@article{ba8498c5970a49eab65308b1b7fa498f,

title = "Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity",

abstract = "Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2-4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo.",

keywords = "Arabidopsis thaliana, Chimeric gene, Nitrate, Nitrate reductase, Over- expression, Protein content",

author = "Ali Nejidat and Genfa Zhang and Michal Grinberg and Heimer, {Yair M.}",

note = "Funding Information: Ali Nejidat was supported by a Deichman Fellowship. Genfa Zhang was a postdoctorate fellow of The Jacob Blaustein International Center. The authors thank Prof. Rachel Nechushtai, for supplying antibodies against Lhcb protein, and Dr. Elliott Birnbaum for editing the manuscript. This research was supported in part by a grant from The Moriah Foundation.",

year = "1997",

month = dec,

day = "5",

doi = "10.1016/S0168-9452(97)00203-3",

language = "English",

volume = "130",

pages = "41--49",

journal = "Plant Science",

issn = "0168-9452",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity

AU - Nejidat, Ali

AU - Zhang, Genfa

AU - Grinberg, Michal

AU - Heimer, Yair M.

N1 - Funding Information: Ali Nejidat was supported by a Deichman Fellowship. Genfa Zhang was a postdoctorate fellow of The Jacob Blaustein International Center. The authors thank Prof. Rachel Nechushtai, for supplying antibodies against Lhcb protein, and Dr. Elliott Birnbaum for editing the manuscript. This research was supported in part by a grant from The Moriah Foundation.

PY - 1997/12/5

Y1 - 1997/12/5

N2 - Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2-4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo.

AB - Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2-4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo.

KW - Arabidopsis thaliana

KW - Chimeric gene

KW - Nitrate

KW - Nitrate reductase

KW - Over- expression

KW - Protein content

UR - http://www.scopus.com/inward/record.url?scp=0031555282&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(97)00203-3

DO - 10.1016/S0168-9452(97)00203-3

M3 - Article

AN - SCOPUS:0031555282

SN - 0168-9452

VL - 130

SP - 41

EP - 49

JO - Plant Science

JF - Plant Science

IS - 1

ER -

Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this