Increased protein content in transgenic Arabidopsis thaliana over- expressing nitrate reductase activity

Ali Nejidat, Genfa Zhang, Michal Grinberg, Yair M. Heimer

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Arabidopsis thaliana was engineered to over-express nitrate reductase (NR) by virtue of the light inducible chimeric gene Lhcb1*3::Nia1*2. The transgenic lines obtained displayed NR activity 2-4 times the level in the wild type, depending on the line. While not displaying advantage with respect to fresh or dry weight, 7-day-old transgenic seedlings did show up to 200% higher protein content than the wild type when grown on solid medium. Up to 30% increase in protein content was also obtained when grown in peat moss for at least 3 weeks. The increase in protein content was evident in several protein bands but was most apparent in that of the large subunit of RuBisCo.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalPlant Science
Volume130
Issue number1
DOIs
StatePublished - 5 Dec 1997

Keywords

  • Arabidopsis thaliana
  • Chimeric gene
  • Nitrate
  • Nitrate reductase
  • Over- expression
  • Protein content

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