Induced color change of conjugated polymeric vesicles by interfacial catalysis of phospholipase A2

Sheldon Yoshio Okada, Raz Jelinek, Deborah Charych

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

A blue to red color change is induced on addition of phospholipase A2 to modified PDA vesicles 1 (PDA = polydiacetylene). This bathochromic transition results from chemical modification of the vesicles by hydrolysis of the enzyme substrate embedded in the PDA matrix. Addition of a known phospholipase inhibitor or removal of Ca2+ ions suppresses the color change, which suggests the potential for applications in high-throughput screening assays.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalAngewandte Chemie - International Edition
Volume38
Issue number5
DOIs
StatePublished - 1 Mar 1999

Keywords

  • Biosensors
  • Conjugation
  • Enzyme catalysis
  • Enzyme inhibitors
  • Vesicles

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry

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