Abstract
A blue to red color change is induced on addition of phospholipase A2 to modified PDA vesicles 1 (PDA = polydiacetylene). This bathochromic transition results from chemical modification of the vesicles by hydrolysis of the enzyme substrate embedded in the PDA matrix. Addition of a known phospholipase inhibitor or removal of Ca2+ ions suppresses the color change, which suggests the potential for applications in high-throughput screening assays.
Original language | English |
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Pages (from-to) | 655-659 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 38 |
Issue number | 5 |
DOIs | |
State | Published - 1 Mar 1999 |
Keywords
- Biosensors
- Conjugation
- Enzyme catalysis
- Enzyme inhibitors
- Vesicles
ASJC Scopus subject areas
- Catalysis
- General Chemistry