Induced pluripotent stem cell-derived cardiomyocytes: generation and enrichment protocols, immature and mature structure and function

Danielle Regev, Polina Baskin, Irit Dolgopyat, Mor Davidor, Fatemeh Kermani, Nina D. Ullrich, Ofer Binah

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Until several years ago, for in-vitro studies scientists have mostly employed animal-derived cardiomyocytes to investigate the following: (1) the excitation-contraction-coupling (ECC) machinery; (2) The effects of disease-related pathological conditions on the ECC machinery; (3) Cardiomyocyte bioenergetics and metabolism; (4) Developmental changes in the ECC machinery in cardiomyocytes obtained from animals at different ages; (5) Inotropic and chronotropic modulation by autonomic agonists and antagonists; (6) Arrhythmia models and antiarrhythmic efficacies of different drugs; (7) Cardiac toxicity of approved drugs and new chemical entities. Although these experimental models have vastly expanded our knowledge regarding cardiac function under physiological and pathophysiological conditions, in many cases, key ECC characteristics of these animal models differ from those of the human heart.

Original languageEnglish
Title of host publicationRecent Advances in iPSC-Derived Cell Types
Subtitle of host publicationVolume 4 in Advances in Stem Cell Biology
PublisherElsevier
Pages191-226
Number of pages36
ISBN (Electronic)9780128222300
DOIs
StatePublished - 1 Jan 2021
Externally publishedYes

Keywords

  • Action potential
  • Differentiation
  • Excitation-contraction-coupling
  • Immature iPSC-CMs
  • Induced pluripotent stem cell (iPSC)
  • Intercellular calcium handling
  • iPSC-derived cardiomyocytes (iPSC-CMs)
  • Maturation of iPSC-CMs
  • Sarcoplasmic reticulum
  • Selection and enrichment of iPSC-CMs

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