Induction and characterization of callus in haloxylon aphyllum (Minkw.) iljin

A. E. Ramirez, E. H. Birnbaum

    Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

    1 Scopus citations


    Callus from mature zygotic embryos of Haloxylon aphyllum was induced on Burnouf- Radosevich-Paupardin (BRP) medium supplemented with different concentrations (0.1, 1.0, 5.0 mg/l) of 2,4-dichlorophenoxyacetic acid (2,4-D), naphthalene acetic acid (NAA), indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) alone or combined with 0.1 mg/l 6-benzyladenine (BA) or kinetin (Kin). The highest frequency of callus induction and greatest amount of callus proliferation occurred on BRP medium supplemented with 1.0 mg/l 2,4-D combined with 0.1 mg/l BA. Following transfer to BRP medium without growth regulators, cell clusters proliferated on the surface of the callus and groups of elongated suspensor cells developed from the clusters. The morphology of the cell clusters and the suspensor-like cells that developed from them support the hypothesis that the tissue is embryogenic callus.

    Original languageEnglish
    Title of host publicationIV International Symposium on In Vitro Culture and Horticultural Breeding
    PublisherInternational Society for Horticultural Science
    Number of pages4
    ISBN (Print)9789066059849
    StatePublished - 1 Jan 2001

    Publication series

    NameActa Horticulturae
    ISSN (Print)0567-7572


    • Auxin
    • Black saxaul
    • Cytokinin
    • Somatic embryogenesis
    • Suspensor

    ASJC Scopus subject areas

    • Horticulture


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