Induction and molecular signature of pathogenic TH17 cells

Emmanuel L Gautier, Tal Shay, Jennifer Miller, Melanie Greter, Claudia Jakubzick, Stoyan Ivanov, Julie Helft, Andrew Chow, Kutlu G Elpek, Simon Gordonov, Amin R Mazloom, Avi Ma'ayan, Wei-Jen Chua, Ted H Hansen, Shannon J Turley, Miriam Merad, Gwendalyn J Randolph, Y Lee, A Awasthi, N YosefF J Quintana, S Xiao, A Peters, C Wu, M Kleinewietfeld, S Kunder, D A Hafler, R A Sobel, A Regev, V K Kuchroo

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

739 Scopus citations

Abstract

We assessed gene expression in tissue macrophages from various mouse organs. The diversity in gene expression among different populations of macrophages was considerable. Only a few hundred mRNA transcripts were selectively expressed by macrophages rather than dendritic cells, and many of these were not present in all macrophages. Nonetheless, well-characterized surface markers, including MerTK and FcγR1 (CD64), along with a cluster of previously unidentified transcripts, were distinctly and universally associated with mature tissue macrophages. TCEF3, C/EBP-α, Bach1 and CREG-1 were among the transcriptional regulators predicted to regulate these core macrophage-associated genes. The mRNA encoding other transcription factors, such as Gata6, was associated with single macrophage populations. We further identified how these transcripts and the proteins they encode facilitated distinguishing macrophages from dendritic cells.
Original languageEnglish
Title of host publicationNature immunology
Pages991-999
Number of pages9
StatePublished - 2012

Publication series

NameNature immunology
Volume13

Keywords

  • Animals
  • Antigens
  • Autoimmune Diseases/immunology
  • CD
  • CD: genetics
  • CD: immunology
  • Cell Diff
  • Cell Differentiation
  • Cell Differentiation/immunology
  • Dendritic Cells
  • Dendritic Cells: cytology
  • Dendritic Cells: immunology
  • Dendritic Cells: metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Genetic
  • Genetic Variation
  • Humans
  • Inflammation/immunology
  • Interleukin-17/biosynthesis
  • Interleukin-23/immunology
  • Interleukin-6/immunology
  • Liver
  • Liver: cytology
  • Liver: immunology
  • Liver: metabolism
  • Lung
  • Lung: cytology
  • Lung: immunology
  • Lung: metabolism
  • Macrophages
  • Macrophages: cytology
  • Macrophages: immunology
  • Macrophages: metabolism
  • Messenger
  • Messenger: genetics
  • Messenger: immunology
  • Mice
  • Microglia
  • Microglia: cytology
  • Microglia: immunology
  • Microglia: metabolism
  • Oligonucleotide Array Sequence Analysis
  • Organ Specificity
  • RNA
  • Spleen
  • Spleen: cytology
  • Spleen: immunology
  • Spleen: metabolism
  • Th17 Cells/immunology
  • Transcription
  • Transcription Factors
  • Transcription Factors: genetics
  • Transcription Factors: immunology
  • Transforming Growth Factor beta1/immunology/metabo
  • Transforming Growth Factor beta3/immunology/metabo

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