TY - JOUR
T1 - Induction of anti-GM1 ganglioside antibodies by Campylobacter jejuni lipopolysaccharides
AU - Wirguin, Itzhak
AU - Briani, Chiara
AU - Suturkova-Milosevic, Ljubica
AU - Fisher, Tom
AU - Della-Latta, Phyllis
AU - Chalif, Peter
AU - Latov, Norman
N1 - Funding Information:
Supported in part by a grant from the Hebrew University-Hadassah Joint Fund, Jerusalem, Israel and by grants from the NIH (WS11766) and from the William Rosenwald Family Fund. The authors are indebted to C. Sicsic and R. Mizrahi-Kol for excellent technical assistance and to Dr. N. Goldfield for editorial suggestions.
PY - 1997/9/1
Y1 - 1997/9/1
N2 - A frequent association exists between acute motor neuropathy, antecedent Campylobacter jejuni (CJ) and anti-GM1 ganglioside antibodies. Despite the chemical and immunological similarity between CJ lipopolysaccharides (LPS) and GM1, the mechanism of induction of anti-GM1 antibodies is still unclear. We used CJ LPS to immunize rats, mice and immunodeficient mice lacking in NK, CD8 + or T-cell populations. None of these animals developed significant anti-GM1 titers. However, rats immunized with keyhole limpet hemocyanin which contains the cross-reactive sugar epitope Gal(β1-3)GalNAc developed high titers of IgM anti-GM1 antibodies. This occurred only after these rats were given an intraperitoneal injection of CJ LPS. These results suggest that a glycoprotein antigenic stimulus can induce B-cells which are autoreactive to ganglioside but which remain anergic. A second stimulus with a cross-reactive LPS can then overcome the anergy to induce autoantibody production. A similar mechanism may explain the occurrence of GM1 antibodies in patients after CJ enteritis.
AB - A frequent association exists between acute motor neuropathy, antecedent Campylobacter jejuni (CJ) and anti-GM1 ganglioside antibodies. Despite the chemical and immunological similarity between CJ lipopolysaccharides (LPS) and GM1, the mechanism of induction of anti-GM1 antibodies is still unclear. We used CJ LPS to immunize rats, mice and immunodeficient mice lacking in NK, CD8 + or T-cell populations. None of these animals developed significant anti-GM1 titers. However, rats immunized with keyhole limpet hemocyanin which contains the cross-reactive sugar epitope Gal(β1-3)GalNAc developed high titers of IgM anti-GM1 antibodies. This occurred only after these rats were given an intraperitoneal injection of CJ LPS. These results suggest that a glycoprotein antigenic stimulus can induce B-cells which are autoreactive to ganglioside but which remain anergic. A second stimulus with a cross-reactive LPS can then overcome the anergy to induce autoantibody production. A similar mechanism may explain the occurrence of GM1 antibodies in patients after CJ enteritis.
KW - Campylobacter jejuni
KW - GM1 ganglioside antibodies
KW - Guillain-Barre syndrome
KW - Lipopolysaccharides
UR - http://www.scopus.com/inward/record.url?scp=0030868254&partnerID=8YFLogxK
U2 - 10.1016/S0165-5728(97)00095-7
DO - 10.1016/S0165-5728(97)00095-7
M3 - Article
C2 - 9307238
AN - SCOPUS:0030868254
SN - 0165-5728
VL - 78
SP - 138
EP - 142
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -